Inter-simple sequence repeat (ISSR)

Genetic variation of Sargassum horneri populations detected by inter-simple sequence repeats

J. R. Ren, Yang, R., He, Y. Y., and Sun, Q. H., Genetic variation of Sargassum horneri populations detected by inter-simple sequence repeats, vol. 14, pp. 619-625, 2015.

The seaweed Sargassum horneri is an important brown alga in the marine environment, and it is an important raw material in the alginate industry. Unfortunately, the fixed resource that was originally reported is now reduced or disappeared, and increased floating populations have been reported in recent years. We sampled a floating population and 4 fixed cultivated populations of S. horneri along the coast of Zhejiang, China.

Genetic diversity of Ceratoides arborescens, a species endemic to China, detected by inter-simple sequence repeat (ISSR)

P. C. Wang, Zhao, L. L., Mo, B. T., Zhang, Y., Chen, J., and Wang, L. B., Genetic diversity of Ceratoides arborescens, a species endemic to China, detected by inter-simple sequence repeat (ISSR), vol. 14, pp. 5658-5666, 2015.

In order to investigate genetic diversity and population structure of Ceratoides arborescens, six populations were selected from different steppe types in Inner Mongolia grasslands of China. Inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among natural populations of C. arborescens. Thirteen ISSR primers generated 154 discernible DNA bands, of which 151 (98.05%) were polymorphic.

Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers

C. Y. Yuan, Zhang, C., Wang, P., Hu, S., Chang, H. P., Xiao, W. J., Lu, X. T., Jiang, S. B., Ye, J. Z., and Guo, X. H., Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers, vol. 13, pp. 3165-3175, 2014.

Okra (Abelmoschus esculentus L.) is not only a nutrient-rich vegetable but also an important medicinal herb. Inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 24 okra genotypes. In this study, the PCR products were separated by electrophoresis on 8% nondenaturing polyacrylamide gel and visualized by silver staining. The 22 ISSR primers produced 289 amplified DNA fragments, and 145 (50%) fragments were polymorphic.

Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers

Y. Li, Wang, S. H., Li, Z. Q., Jin, C. F., and Liu, M. H., Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers, vol. 13, pp. 8704-8713, 2014.

Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among which 89.2, 65.1, and 88.0% of the fragments were polymorphic, respectively. Cluster analysis revealed that Qinzhong No. 3, Xiaoyeci, Qinzhong No. 1, and Qinzhong No.

Genetic diversity and pathogenicity differentiation of Sclerotinia sclerotiorum on rapeseed (Brassica napus L.) in Anhui Province, China

D. F. Xu, Li, X. L., Pan, Y. M., Dai, Y. L., Li, P., Chen, F. X., Zhang, H. J., Guo, M., and Gao, Z. M., Genetic diversity and pathogenicity differentiation of Sclerotinia sclerotiorum on rapeseed (Brassica napus L.) in Anhui Province, China, vol. 13, pp. 10704-10713, 2014.

The pathogenicity of 47 isolates of Sclerotinia sclerotiorum from oilseed rape (Brassica napus L.) in Anhui, China, was tested by detached leaf inoculation using the susceptible rape cultivar Wanyou-14. All isolates were pathogenic to the cultivar and could be grouped into 3 categories based on the lesion length on the leaves tested: weak pathogenicity type, intermediate pathogenicity type, and strong pathogenicity type. This suggested that there was differentiation in the pathogenicity among the strains tested of S. sclerotiorum.

Genetic diversity of Cosmos species revealed by RAPD and ISSR markers

A. Rodríguez-Bernal, Piña-Escutia, J. L., Vázquez-García, L. M., and Arzate-Fernández, A. M., Genetic diversity of Cosmos species revealed by RAPD and ISSR markers, vol. 12, pp. 6257-6267, 2013.

The genus Cosmos is native of America and is constituted by 34 species; 28 of them are endemic of Mexico. The cosmos are used as a nematicide, antimalarial, and antioxidative agent. The aim of this study was to estimate the genetic diversity among 7 cosmos species based on random amplified polymorphic DNA (RAPD) and inter-simple sequences repeats (ISSR) markers.

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