Inter-simple sequence repeat

Genetic diversity and taxonomic status of Pinus tabulaeformis f. shekanensis revealed by ISSR markers

Z. - H. Liu, Xie, Q., and Li, Z. - Q., “Genetic diversity and taxonomic status of Pinus tabulaeformis f. shekanensis revealed by ISSR markers”, vol. 14, pp. 1034-1043, 2015.

Pinus tabulaeformis f. shekanensis is a rare taxon endemic in the Ziwuling Loess Plateau, of which only one population is known. Inter-simple sequence repeat molecular markers were employed to compare the taxon’s genetic diversity with its 4 nearest wild relatives (P. tabulaeformis, P. tabulaeformis var. mukdensis, P. massoniana, and P. henryi) to assess the taxonomic status of P. tabulaeformis f. shekanensis.

SCAR marker for sex identification of Pistacia chinensis Bunge (Anacardiaceae)

Q. Sun, Yang, X., and Li, R., “SCAR marker for sex identification of Pistacia chinensis Bunge (Anacardiaceae)”, vol. 13, pp. 1395-1401, 2014.

Pistacia chinensis Bunge is a dioecious plant that originated in China, and its sex cannot be identified at the early stage of cultivation by only its appearance. Recent studies show that the seed of P. chinensis is an ideal feedstock for biofuel production. To guide the cultivation of this energy plant scientifically, a new method is urgently needed to identify the sex of P. chinensis seedlings.

Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants

A. Wannajindaporn, Poolsawat, O., Chaowiset, W., and Tantasawat, P. A., “Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants”, vol. 13, pp. 5333-5342, 2014.

In vitro mutagenesis of Dendrobium ‘Earsakul’ was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymorphic fragments were produced by 9 of 12 ISSR primers. A total of 173 amplified ISSR fragments varying in size from 140 to 5000 bp were obtained, 39 of which were polymorphic (22.5%).

Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers

G. F. Cui, Wu, L. F., Wang, X. N., Jia, W. J., Duan, Q., Ma, L. L., Jiang, Y. L., and Wang, J. H., “Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers”, vol. 13, pp. 5778-5786, 2014.

Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average of 13 bands were amplified by each primer. Two software packages, POPGENE 1.32 and NTSYSpc 2.1, were used to analyze the data matrix.

Genetic diversity and population structure of wild Dipsacus asperoides in China as indicated by ISSR markers

D. X. Chen, Li, L. Y., Zhang, X., Wang, Y., and Zhang, Z., “Genetic diversity and population structure of wild Dipsacus asperoides in China as indicated by ISSR markers”, vol. 13, pp. 6340-6349, 2014.

In order to evaluate the genetic diversity and genetic structure of wild Dipsacus asperoides, we surveyed genetic polymorphisms in 288 individuals from 12 populations using ISSR. A total of 240 bands were amplified, among which 190 were polymorphic loci. At the species level, genetic diversity was found to be abundant: PPB = 79.17%, NE = 1.2152, H = 0.1361, and Hsp = 0.2213.

Comparative analysis of genetic diversity among species of Chrysanthemum and its related genera using inter-simple sequence repeat and sequence-related amplified polymorphism markers

X. Zhang, Zhang, F., Zhao, H., Guan, Z., Chen, S., Jiang, J., Fang, W., and Chen, F., “Comparative analysis of genetic diversity among species of Chrysanthemum and its related genera using inter-simple sequence repeat and sequence-related amplified polymorphism markers”, vol. 13, pp. 8469-8479, 2014.

In this study, inter-simple sequence repeats (ISSRs) and sequence-related amplified polymorphism (SRAP) were applied to assess the genetic diversity in 38 species of Chrysanthemum and related genera. A total of 204 and 567 bands were amplified by 24 ISSR and 25 SRAP primers, of which 196 (97%) and 557 (99%) were polymorphic, respectively. The ISSR-based genetic similarity ranged from 0.016 to 0.886 and averaged 0.201, while the SRAP-based genetic similarity varied from 0.010 to 0.811 and averaged 0.122.

Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis

S. F. Chai, Jiang, Y. S., Zhuang, X. Y., Shi, Y. C., Wei, X., Luo, W. H., and Chen, Z. Y., “Genetic diversity and differentiation of the endangered and endemic species Sauvagesia rhodoleuca in China as detected by ISSR analysis”, vol. 13, pp. 8258-8267, 2014.

Sauvagesia rhodoleuca (Ochnaceae) is an endangered plant that is endemic to southern China. The levels of genetic variation and patterns of population structure in S. rhodoleuca were investigated using inter-simple sequence repeat markers. Eleven primers were used to amplify DNA samples from 117 individuals, and a total of 92 loci were detected.

Selection of sugar cane full-sib families using mixed models and ISSR markers

L. M. Almeida, Viana, A. P., Gonçalves, G. M., and Entringer, G. C., “Selection of sugar cane full-sib families using mixed models and ISSR markers”, vol. 13, pp. 9202-9212, 2014.

In 2006, an experiment examining families belonging to the first selection stage of the Sugar Cane Breeding Program of Universidade Federal Rural do Rio de Janeiro/Rede Interuniversitária para o Desenvolvimento do Setor Sucroalcooleiro was conducted. Families and plants within families were evaluated to select superior plants for subsequent stages of the breeding program. The experiment was arranged in a randomized block design, in which progenies were grouped into 4 sets, each with 4 replicates and 100 seedlings per plot.

Analysis of the genetic diversity of Chinese native Cannabis sativa cultivars by using ISSR and chromosome markers

L. G. Zhang, Chang, Y., Zhang, X. F., Guan, F. Z., Yuan, H. M., Yu, Y., and Zhao, L. J., “Analysis of the genetic diversity of Chinese native Cannabis sativa cultivars by using ISSR and chromosome markers”, vol. 13, pp. 10490-10500, 2014.

Hemp (Cannabis sativa) is an important fiber crop, and native cultivars exist widely throughout China. In the present study, we analyzed the genetic diversity of 27 important Chinese native hemp cultivars, by using inter-simple sequence repeats (ISSR) and chromosome markers. We determined the following chromosome formulas: 2n = 20 = 14m + 6sm; 2n = 20 = 20m; 2n = 20 = 18m + 2sm; 2n = 20 = 16m + 4sm; and 2n = 20 = 12m + 8sm.

Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

P. Khajudparn, Prajongjai, T., Poolsawat, O., and Tantasawat, P. A., “Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)”, vol. 11, pp. 3329-3338, 2012.

Mungbean improvement via hybridization requires the identification of true F1 hybrids from controlled crosses before further generations of selfing/crossing and selection. We utilized inter-simple sequence repeat (ISSR) markers for identifying putative F1 hybrids from six cross combinations whose morphological characteristics were very similar to those of their respective female parents and could not be visually discriminated from the self-pollinated progeny.

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