Inducible promoter

A new inducible expression system in a transformed green alga, Chlorella vulgaris

Y. F. Niu, Zhang, M. H., Xie, W. H., Li, J. N., Gao, Y. F., Yang, W. D., Liu, J. S., and Li, H. Y., A new inducible expression system in a transformed green alga, Chlorella vulgaris, vol. 10, pp. 3427-3434, 2011.

Genetic transformation is useful for basic research and applied biotechnology. However, genetic transformation of microalgae is usually quite difficult due to the technical limitations of existing methods. We cloned the promoter and terminator of the nitrate reductase gene from the microalga Phaeodactylum tricornutum and used them for optimization of a transformation system of the microalga Chlorella vulgaris. This species has been used for food production and is a promising candidate as a bioreactor for large-scale production of value-added proteins.

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