Induced pluripotent stem cells

Determination of the potential of induced pluripotent stem cells to differentiate into mouse nucleus pulposus cells in vitro

K. Liu, Chen, Z., Luo, X. W., Song, G. Q., Wang, P., Li, X. D., Zhao, M., Han, X. W., Bai, Y. G., Yang, Z. L., and Feng, G., “Determination of the potential of induced pluripotent stem cells to differentiate into mouse nucleus pulposus cells in vitro”, vol. 14, pp. 12394-12405, 2015.

We determined the potential for induced pluripotent stem (iPS) cells to differentiate into nucleus pulposus (NP)-like cells in mice. iPS cells were generated from tail-tip fibroblasts. We used a pellet culture model with the aim of determining the applicability of iPS cell-based therapy to intervertebral disc degeneration (IVD). The cell pellet was cultured in an NP cell basal medium comprising Dulbecco’s modified Eagle’s medium supplemented with transforming growth factor beta 1, dexamethasone, ascorbate-2-phosphate, and 1% ITS-Premix.

Generation of induced pluripotent mouse stem cells in an indirect co-culture system

F. L. Dong, Kaleri, H. A., Lu, Y. D., Song, C. L., Jiang, B. C., Zhang, B. L., Wang, L. J., Wang, X. G., Ma, X. S., Wu, B. J., Song, H., Li, J., and Liu, H. L., “Generation of induced pluripotent mouse stem cells in an indirect co-culture system”, vol. 11, pp. 4179-4186, 2012.

Typically, production of induced pluripotent stem cells requires direct contact with feeder cells. However, once the stem cells have reached the appropriate maturation point, it is difficult to separate them from feeder cells, which must be irradiated with γ-rays or treated with the antibiotic mitomycin-C. We used a microporous poly-membrane-based indirect contact co-culture system with mouse embryonic fibroblasts to induce mouse pluripotent stem cells without radiation or antibiotics.

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