Indirect immunofluorescence

Expression of CD44 in sheep oocytes and preimplantation embryos

J. V. Luz, Alcântara-Neto, A. S., Batista, R. I. T. P., Souza, J. M. G., Teixeira, D. I. A., Melo, L. M., and Freitas, V. J. F., Expression of CD44 in sheep oocytes and preimplantation embryos, vol. 11, pp. 799-809, 2012.

The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35) and embryos (N = 41) were obtained by laparotomy from adult hair ewes submitted to ovarian stimulation treatment.

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