Imprinted genes

Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes

F. V. M. Rubatino, Carobin, N. V., Freitas, M. L., de Oliveira, V. T., Pietra, R. X., Oliveira, P. P. R., Bosco, A. A., and Jehee, F. S., Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes, vol. 14, pp. 7864-7872, 2015.

High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, primers that include CpG site(s) and are fully complementary to the methylated sequence have been proposed.

Expression and significance of the imprinted gene PEG10 in placenta of patients with preeclampsia

X. Y. Liang, Chen, X., Jin, Y. Z., Chen, X. O., and Chen, Q. Z., Expression and significance of the imprinted gene PEG10 in placenta of patients with preeclampsia, vol. 13, pp. 10607-10614, 2014.

The aim of this study was to investigate the expression and significance of the imprinted gene PEG10 (paternally expressed gene 10) in preeclampsia placental tissue. Quantitative real-time reverse transcriptase polymerase chain reaction and immunohistochemistry to evaluate mRNA and protein expression and distribution of PEG10 in placental tissues obtained from 22 preeclampsia patients (8 patients with mild preeclampsia, 14 cases of severe preeclampsia). At the same time, 22 cases of normal pregnant women served as controls.

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