Immunohistochemistry

Immunohistochemical and molecular analysis of caveolin-1 expression in canine mammary tumors

D. A. P. C. Zuccari, Castro, R., Gavioli, A. F., Mancini, U. M., Frade, C. S., and Leonel, C., “Immunohistochemical and molecular analysis of caveolin-1 expression in canine mammary tumors”, vol. 11, pp. 153-165, 2012.

Caveolin-1 (Cav-1) is a structural protein present in invaginations of the cell membrane. In human breast cancer, the cav-1 gene is believed to be a tumor suppressor gene associated with inhibition of tumor metastasis. However, little is known about its expression, regulation and function in canine mammary tumors. Expression levels of cav-1 were investigated using real-time PCR and immunohistochemical detection with an anti-human Cav-1 antibody. Gene expression stability of different samples was analyzed using the geNorm software.

Expressions of Src homology 2 domain-containing phosphatase and its clinical significance in laryngeal carcinoma

L. B. Dong, Li, G. Q., Tian, Z. H., Wang, Z. M., and Xu, K., “Expressions of Src homology 2 domain-containing phosphatase and its clinical significance in laryngeal carcinoma”, vol. 12, pp. 4207-4212, 2013.

We investigated the expression of Src homology 2 domain-containing phosphatase (SHP-2) in laryngeal carcinoma and its clinical significance. Expression of SHP-2 was detected by immunohistochemical staining in normal mucosal tissues and various grades of laryngeal carcinoma. We looked for possible correlations between expression of SHP-2 in laryngeal carcinoma and clinical staging and lymph node metastasis. Immunochemical staining results revealed that the SHP-2 expression was significantly higher (88.24%) in laryngeal carcinoma than in normal mucosal tissue (25%).

Association of chemotactic factor receptor 5 gene with breast cancer

Y. Zhang, Meng, F. Y., Li, W. L., Zhou, C. X., Guan, Z., and Fan, H. Y., “Association of chemotactic factor receptor 5 gene with breast cancer”, vol. 12, pp. 5289-5300, 2013.

We designed a 2-stage study to investigate chemotactic factor receptor 5 (CCR5) gene expression in breast cancer tissues and axillary lymph nodes and analyze the association between the CCR5-Δ32 gene polymorphism and the clinical features and prognosis of breast cancer patients. The first stage examined 72 cases of invasive ductal carcinoma and axillary lymph node tissue, 50 cases of breast fibroadenoma tissue, and 40 cases of normal breast tissue.

Interleukin-8 expression associated with canine mammary tumors

D. A. P. C. Zuccari, Castro, R., Gelaleti, G. B., and Mancini, U. M., “Interleukin-8 expression associated with canine mammary tumors”, vol. 10, pp. 1522-1532, 2011.

The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR.

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