Hygromycin phosphotransferase gene

Optimization of factors affecting Agrobacterium-mediated transformation of Micro-Tom tomatoes

M. Guo, Zhang, Y. L., Meng, Z. J., and Jiang, J., Optimization of factors affecting Agrobacterium-mediated transformation of Micro-Tom tomatoes, vol. 11, pp. 661-671, 2012.

Micro-Tom is the smallest known variety of tomatoes. An orthogonal experimental design L16 (45) was used to optimize Agrobacterium-mediated transformation of cotyledon explants of Lycopersicon esculentum cv. Micro-Tom. Four parameters were investigated to determine their effect on transformation frequency: the concentration of bacterial suspension, time of dip in bacterial suspension, co-cultivation time, and concentration of carbenicillin.

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