Hygromycin

Stable expression and integrated hepatitis B virus genome in a human hepatoma cell line

L. Y. Wang, Li, Y. G., Chen, K., Li, K., Qu, J. L., Qin, D. D., and Tang, H., Stable expression and integrated hepatitis B virus genome in a human hepatoma cell line, vol. 11, pp. 1442-1448, 2012.

HepG2.2.15 cell is a widely used cell model for studying HBV (hepatitis B virus) in vitro. In these cells, the HBV genome is integrated in several sites of HepG2 cellular DNA. These multiple copies may have some influence on the cellular processes. We constructed a new plasmid, pSEH-Flag-HBV, and transfected it into HepG2 cells, and then screened it with hygromycin. We then used ELISA, PCR, and RT-PCR to detect the expression of HBV in these cell lines. A cell line that stably expressed hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) was established.

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