Human gastric epithelial cells

Effects of BCL2 transfection on the cell cycle and proliferation of human GES-1 cells

G. Y. Shi, Zhao, L. L., Zhang, K., Zhou, H. X., Liu, A. H., Li, J., Li, G., and Zhu, L. H., Effects of BCL2 transfection on the cell cycle and proliferation of human GES-1 cells, vol. 14, pp. 12022-12029, 2015.

We investigated the effects of BCL2 transfection on the cell cycle and proliferation of GES-1 cells. A pcDNA3-BCL2 plasmid was used to transfect GES-1 cell line human gastric epithelial cells. Clones were obtained by G418 screening. BCL2-positive cells were identified by fluorescence immunohistochemistry. The pcDNA3-BCL2 vectors carrying the NeoR gene were transfected into GES-1 cells, while the empty plasmid was transfected into the same cells as controls. BCL2-positive clones were screened by neomycin 418 (G418).

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