Homology modeling

Molecular cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene from Penicillium expansum PE-12

T. Zhang, Qi, Z., Yu, Q. S., and Tang, K. X., “Molecular cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene from Penicillium expansum PE-12”, vol. 12, pp. 2442-2454, 2013.

Penicillium expansum produces large amounts of lipase, which is widely used in laundry detergent and leather industry. We isolated the glyceraldehyde-3-phosphate dehydrogenase gene (PeGPD) from P. expansum PE-12 through reverse transcriptase PCR and 5ꞌ-3ꞌ-rapid amplification of cDNA ends (RACE-PCR). The gene is 1266 bp long, including an ORF of 1014 bp, encoding a polypeptide chain of 337 amino acids. A phylogenetic tree based on GPD proteins showed that P. expansum is close to Aspergillus species, but comparatively distant from P.

Anthranilate synthase subunit organization in Chromobacterium violaceum

C. A. Carminatti, Oliveira, I. L., Recouvreux, D. O. S., Antônio, R. V., and Porto, L. M., “Anthranilate synthase subunit organization in Chromobacterium violaceum”, vol. 7, pp. 830-838, 2008.

Tryptophan is an aromatic amino acid used for protein synthesis and cellular growth. Chromobacterium violaceum ATCC 12472 uses two tryptophan molecules to synthesize violacein, a secondary metabolite of pharmacological interest. The genome analysis of this bacterium revealed that the genes trpA-F and pabA-B encode the enzymes of the tryptophan pathway in which the first reaction is the conversion of chorismate to anthranilate by anthranilate synthase (AS), an enzyme complex. In the present study, the organization and structure of AS protein subunits from C.

The olfactory receptor universe - from whole genome analysis to structure and evolution

T. Olender, Feldmesser, E., Atarot, T., Eisenstein, M., and Lancet, D., “The olfactory receptor universe - from whole genome analysis to structure and evolution”, vol. 3, pp. 545-553, 2004.

Olfactory receptors (ORs) constitute the largest gene-family in the vertebrate genome. We have attempted to provide a comprehensive view of the OR universe through diverse tools of bioinformatics and computational biology. Among others, we have constructed the Human Olfactory Receptor Data Exploratorium (HORDE, http://bioportal.weizmann.ac.il/HORDE/) as a free online resource, which integrates information on ORs from different species.

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