Homologue of Serpina3n

Cloning and prokaryotic expression of rat homolog of Serpina3n and its expression change during liver regeneration

G. P. Wang, Zhang, X. S., Li, Y. H., Zheng, J. L., Tang, C. Z., and Zhang, W. X., Cloning and prokaryotic expression of rat homolog of Serpina3n and its expression change during liver regeneration, vol. 11, pp. 3175-3185, 2012.

A strikingly upregulated expressed sequence tag was screened from regenerating rat liver at 8 h in a 0-4-8-12 h short-interval successive partial hepatectomy model from a previous study. In the present study, a full-length open reading frame (ORF) corresponding to this expressed sequence tag was predicted through electronic cloning and was subsequently cloned from an 8-h rat regenerating liver and deposited in GenBank (accession No. HM448398). Sequence analysis of HM448398 and the predicted ORF revealed that the two ORFs may be different transcripts of a gene.

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