Holothuria leucospilota

Development and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria leucospilota

G. Dai, Li, Z. B., Shangguan, J. B., Ning, Y. F., Deng, H. W., Yuan, Y., Huang, Y. S., Yang, H., and Lu, J., “Development and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria leucospilota”, vol. 14, pp. 538-541, 2015.

Holothuria leucospilota is a tropical holothurian species that is widely distributed in the tropical and sub-tropical India-Western Pacific Region. Eight polymorphic microsatellite loci were developed from H. leucospilota by using the protocol fast isolation by amplified fragment length polymorphism of sequences containing repeats and tested in 30 individuals from Hainan Island in China. The number of alleles was 2-6 and polymorphism information content ranged from 0.371-0.694.

Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota

J. B. Shangguan, Li, Z. B., Ning, Y. F., Huang, Y. S., Yuan, Y., Lu, J., Li, B. B., and Mao, X. Q., “Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota”, vol. 14, pp. 6555-6560, 2015.

The sea cucumber Holothuria leucospilota has high me­dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individu­als from a wild population.

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