High-resolution melting

Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep

M. Yang, Yue, Y. J., Guo, T. T., Han, J. L., Liu, J. B., Guo, J., Sun, X. P., Feng, R. L., Wu, Y. Y., Wang, C. F., Wang, L. P., and Yang, B. H., Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep, vol. 13, pp. 2645-2653, 2014.

Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity and accuracy. In this report, we assessed the latest of the non-gel-based methods, high-resolution melting (HRM) curve analysis.

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