An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers
“An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers”, vol. 15, p. -, 2016.
, Random amplified polymorphic DNA (RAPD) is a widely used molecular marker technique. As traditional RAPD has poor reproducibility and productivity, we previously developed an improved RAPD method (termed RAMP-PCR), which increased the reproducibility, number of bands, and efficiency of studies on polymorphism. To further develop the efficiency of this method, we used high-GC content primers for improved RAMP-PCR with DNA samples from Lonicera japonica.