Hevea brasiliensis

Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in Hevea brasiliensis

W. Zhai, Zhao, Y., Zhang, L. X., and Li, X. J., Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in Hevea brasiliensis, vol. 13, pp. 4348-4360, 2014.

The tomato Pto gene encodes a serine/threonine kinase (STK) whose molecular characterization has provided valuable insights into the disease resistance mechanism of tomato. Therefore, Pto is considered as a promising candidate for engineering broad-spectrum pathogen resistance in this crop. In this study, a pair of degenerate primers based on conserved subdomains of plant STKs similar to the tomato Pto protein was used to amplify similar sequences in a hevea cultivar (Hevea brasiliensis Muell. Arg).

Cloning and molecular characterization of a cDNA encoding a small GTPase from Hevea brasiliensis

H. L. Li, Guo, D., Tian, W. M., and Peng, S. Q., Cloning and molecular characterization of a cDNA encoding a small GTPase from Hevea brasiliensis, vol. 12, pp. 3305-3313, 2013.

Small GTPases play a critical role in the regulation of a range of cellular processes including growth, differentiation, and intracellular transportation. The cDNA encoding a small GTPase, designated as HbGTPase1, was isolated from Hevea brasiliensis. HbGTPase1 was 882 bp long containing a 612-bp open reading frame encoding a putative protein of 203 amino acids, flanked by an 83-bp 5'-untranslated region (UTR) and a 187-bp 3'-UTR. The predicted molecular mass of HbGTPase1 is 22.62 kDa, with an isoelectric point of 5.06.

Development and characterization of novel expressed sequence tag-derived simple sequence repeat markers in Hevea brasiliensis (rubber tree)

Z. W. An, Li, Y. C., Zhai, Q. L., Xie, L. L., Zhao, Y. H., and Huang, H. S., Development and characterization of novel expressed sequence tag-derived simple sequence repeat markers in Hevea brasiliensis (rubber tree), vol. 12, pp. 5905-5910, 2013.

Cultivated clones of Hevea brasiliensis have a narrow genetic base. In order to broaden the genetic base, it is first necessary to investigate the genetic diversity of wild populations. Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed to investigate the genetic diversity of Hevea populations. Four hundred and thirty microsatellites were identified and 148 primers were designed to amplify the loci. Twenty-nine primer pairs were synthesized and evaluated for their ability to detect genetic polymorphisms among 40 wild accessions of H.

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