Heteroduplex DNA

Identification and analysis of a 5-bp indel of a porcine BMP7 gene promoter

P. Lu, Feng, X., Zhou, J. S., Liu, X. B., Chen, Q. X., and Li, M., Identification and analysis of a 5-bp indel of a porcine BMP7 gene promoter, vol. 13, pp. 4326-4335, 2014.

Bone morphological protein7 (BMP7), a member of the transforming growth factor-β (TGF-β) family, was first identified because of its ability to induce ectopic chondro-osteogenesis in vivo. It also plays a crucial role in the growth, development, and physiological functioning of the reproductive system. Among intra-ovarian growth factors, many studies have shown that BMP7 plays a pivotal role in regulating the early phases of follicular growth.

Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes

P. A. Roratto, Bartholomei-Santos, M. L., Gutierrez, A. M., Kamenetzky, L., Rosenzvit, M. C., and Zaha, A., Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes, vol. 5, pp. 542-552, 2006.

Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobility in polyacrylamide gel electrophoresis. Heteroduplex analysis was used to determine if the U1 snRNA microsatellite could be a useful genetic marker in Echinococcus granulosus. A U1 snRNA microsatellite fragment from E.

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