Hepatitis B virus S protein small hairpin RNA

Construction and functional identification of a hepatitis B virus S protein small hairpin RNA recombinant adenovirus

S. Wang, Liu, Y. Z., Qin, D. D., Zou, C. C., Sheng, Y. R., Huang, A. L., and Tang, H., Construction and functional identification of a hepatitis B virus S protein small hairpin RNA recombinant adenovirus, vol. 13, pp. 3495-3501, 2014.

Hepatitis B virus S protein (HBs) plays an important role in hepatocellular carcinoma progression. However, to date, no direct and effective methods exist to research the function of HBs. Here, we combined the technology of RNA interference with recombinant adenovirus, constructed a recombinant adenovirus-expressing small hairpin RNA of HBs, and infected HepG2.2.15 cells. Then, reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time PCR, enzyme-linked immunosorbent assay, and Western blot analysis were performed to verify the interference effects.

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