Hepatitis B virus

Polymorphism of UGT1A1*28 (TA)7 and liver damage in hepatitis B virus-positive patients in Albania

E. Marku, Maltese, P. E., Koni, M., Capodicasa, N., Qendro, I. S., Rigoni, E., Cecchin, S., and Bertelli, M., Polymorphism of UGT1A1*28 (TA)7 and liver damage in hepatitis B virus-positive patients in Albania, vol. 14, pp. 5221-5228, 2015.

Hepatitis B virus (HBV) is the infectious agent of both acute and chronic hepatitis. HBV exists in multiple genotypic variants that differ in their capacity to become persistent chronic infections and in their clinical manifestations, including hepatocellular carcinoma. The 8 genotypes (A-H) of HBV show a specific worldwide geographic distribution and are correlated with different disease course, severity, and response to therapy.

 Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro

Y. - B. Deng, Qin, H. - J., Luo, Y. - H., Liang, Z. - R., and Zou, J. - J.,  Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro, vol. 14, pp. 5445-5451, 2015.

The aim of this study was to investigate the effects of inhibition of the hepatitis B virus (HBV) S gene by polypurine region locked nucleic acid on viral replication in cells. We designed and synthe­sized a locked nucleic acid, phosphorothioate oligonucleotides, unmod­ified oligonucleotides, and unrelated control sequence for the hepatitis B virus S gene polypurine region.

Association of the IFNAR1-17470 and IL-10-592 cytokine variants with susceptibility to chronic hepatitis B viral infections in a Chinese population

Y. Xiang, Huang, S. F., Xia, J. R., Ye, D. Q., Chen, P., Yang, S. S., Sun, S., Lai, X. F., and Zhang, L. P., Association of the IFNAR1-17470 and IL-10-592 cytokine variants with susceptibility to chronic hepatitis B viral infections in a Chinese population, vol. 13, pp. 9187-9195, 2014.

An association between the sequence variants of cytokine genes and various clinical outcomes in subjects infected with the hepatitis B virus (HBV) has been demonstrated. However, the results are inconsistent and inconclusive. Further studies in other populations and the evaluation of a greater number of individuals may contribute to a better understanding of the influence of the cytokine genetic variants on the evolution of HBV infections.

An improved reverse dot hybridization for simple and rapid detection of adefovir dipivoxil-resistant hepatitis B virus

Y. Hu, Zhang, W. L., Xie, S. L., Zhao, Y., Hu, J. L., Cai, X. F., Lai, G. Q., and Huang, A. L., An improved reverse dot hybridization for simple and rapid detection of adefovir dipivoxil-resistant hepatitis B virus, vol. 11, pp. 53-60, 2012.

Early detection of adefovir dipivoxil-resistant mutants during long-term treatment of chronic hepatitis B virus (HBV) infection with this drug is of great clinical importance. We developed an improved reverse dot hybridization test for simple and rapid detection of the rtA181V/T and rtN236T mutations associated with adefovir dipivoxil resistance in chronic hepatitis B patients. Probes were designed for genotypes B, C, and D of this resistance characteristic; a total of 70 clinical samples were analyzed with this improved reverse dot hybridization assay.

RANTES gene polymorphisms (-403G>A and -28C>G) associated with hepatitis B virus infection in a Saudi population

A. Al-Qahtani, Alarifi, S., Al-Okail, M., Hussain, Z., Abdo, A., Sanai, F., Al-Anazi, M., Khalaf, N., Al-Humaidan, H., Al-Ahdal, M., and Almajhdi, F. N., RANTES gene polymorphisms (-403G>A and -28C>G) associated with hepatitis B virus infection in a Saudi population, vol. 11, pp. 855-862, 2012.

Besides the host immune response, genetic and environmental factors play crucial roles in the manifestation of hepatitis B virus (HBV) infection. “Regulated on activation normal T-cell expressed and secreted” factor (RANTES) plays a vital role in CD4+, CD8+ T-lymphocyte and dendritic cell activation and proliferation in inflammation. Single nucleotide polymorphisms (SNPs) in the RANTES gene are associated with several viral and non-viral diseases.

Stable expression and integrated hepatitis B virus genome in a human hepatoma cell line

L. Y. Wang, Li, Y. G., Chen, K., Li, K., Qu, J. L., Qin, D. D., and Tang, H., Stable expression and integrated hepatitis B virus genome in a human hepatoma cell line, vol. 11, pp. 1442-1448, 2012.

HepG2.2.15 cell is a widely used cell model for studying HBV (hepatitis B virus) in vitro. In these cells, the HBV genome is integrated in several sites of HepG2 cellular DNA. These multiple copies may have some influence on the cellular processes. We constructed a new plasmid, pSEH-Flag-HBV, and transfected it into HepG2 cells, and then screened it with hygromycin. We then used ELISA, PCR, and RT-PCR to detect the expression of HBV in these cell lines. A cell line that stably expressed hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) was established.

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