Hemoglobinopathies

Abnormal hemoglobin phenotypes in carriers of mild anemia in Latin America

P. J. A. Zamaro and Bonini-Domingos, C. R., Abnormal hemoglobin phenotypes in carriers of mild anemia in Latin America, vol. 9. pp. 425-428, 2010.

We looked for abnormal hemoglobins in blood samples sent for diagnosis of anemia. Identification of the hemoglobins was made using electrophoretic, chromatographic and molecular procedures. The 2020 blood samples were of patients from various regions of Brazil and from some other Latin American countries. Among the abnormal hemoglobins that we found, 3.5% are known to be rare, while 51% had an electrophoretic profile similar to that of Hb S at alkaline pH. Differentiation was possible only by combining electrophoretic and chromatographic methods.

Three-dimensional visualization of human hemoglobin phenotypes with HPLC

L. M. Storti-Melo, Mangonaro, P. H., Valêncio, C. R., Valêncio, C. R., C. Junior, T., and Domingos, C. R. B., Three-dimensional visualization of human hemoglobin phenotypes with HPLC, vol. 8, pp. 354-363, 2009.

Hemoglobinopathies were included in the Brazilian Neonatal Screening Program on June 6, 2001. Automated high-performance liquid chromatography (HPLC) was indicated as one of the diagnostic methods. The amount of information generated by these systems is immense, and the behavior of groups cannot always be observed in individual analyses. Three-dimensional (3-D) visualization techniques can be applied to extract this information, for extracting patterns, trends or relations from the results stored in databases.

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