Heat stress

Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo

H. B. Chen, Zhang, X. C., Cheng, Y. F., Abdelnasir, A., Tang, S., Kemper, N., Hartung, J., and Bao, E. D., “Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo”, vol. 14, pp. 1994-2005, 2015.

To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro.

Effect of heat stress on heat-shock protein (Hsp60) mRNA expression in rainbow trout Oncorhynchus mykiss

H. N. Shi, Liu, Z., Zhang, J. P., Kang, Y. J., Wang, J. F., Huang, J. Q., and Wang, W. M., “Effect of heat stress on heat-shock protein (Hsp60) mRNA expression in rainbow trout Oncorhynchus mykiss”, vol. 14. pp. 5280-5286, 2015.

The enhanced expression of heat shock proteins (hsps) in organisms can be detected in response to many kinds of stressor. For fish, high temperature is an important stressor, and hsp expression is associated with differences in environmental temperature.

Association analysis between SNPs in the 5'-flanking region of the chicken GRP78 gene, thermotolerance parameters, and tissue mRNA expression

L. N. Kong, Zhang, D. X., Ji, C. L., Zhang, X. Q., and Luo, Q. B., “Association analysis between SNPs in the 5'-flanking region of the chicken GRP78 gene, thermotolerance parameters, and tissue mRNA expression”, vol. 14, pp. 6110-6123, 2015.

Glucose-regulated protein 78 (GRP78) is a molecular chaperone in the endoplasmic reticulum and can be induced by different kinds of environmental and physiological stress. Thus far, the role of the GRP78 gene in thermotolerance in chickens has not been investigated.

Expression of heat shock protein 90 alpha (Hsp90α) in primary neonatal rat myocardial cells exposed to various periods of heat stress in vitro

A. Islam, Rehana, B., Zhang, M., Liu, Z. J., Tang, S., Hartung, J., and Bao, E. D., “Expression of heat shock protein 90 alpha (Hsp90α) in primary neonatal rat myocardial cells exposed to various periods of heat stress in vitro”, vol. 13, pp. 2806-2816, 2014.

The objective of this study was to investigate the mechanism of heat shock protein 90 alpha (Hsp90α) protection against heart damage resulting from heat stress by detecting Hsp90α mRNA, Hsp90α protein, protein localization, and cell damage in primary myocardial cells of neonatal rats in response to heat stress in vitro. The cells were heat-stressed at 42°C in an incubator with 95% air and 5% CO2 for different periods.

Mitochondrial gene expression in the liver and muscle of high and low feed efficiency Japanese quail layers subjected to different environmental temperatures

D. M. Voltolini, Del Vesco, A. P., Gasparino, E., Guimarães, S. E. F., Neto, A. R. Oliveira, Batista, E., and Ton, A. P. S., “Mitochondrial gene expression in the liver and muscle of high and low feed efficiency Japanese quail layers subjected to different environmental temperatures”, vol. 13, pp. 4940-4948, 2014.

We evaluated the adenine nucleotide translocator (ANT) and cytochrome oxidase subunit III (COX III) mRNA expressions in the muscle and liver of Japanese quails presenting high and low feed efficiency (FE), and subjected them to three different environmental temperatures: comfort, heat stress (38°C), and cold stress (10°C). ANT mRNA expression was lower in the liver of heat-stressed animals. In the muscle, higher ANT and COX III mRNA expressions were observed in high-FE and cold-stressed animals.

Effects of dietary betaine supplementation subjected to heat stress on milk performances and physiology indices in dairy cow

L. Zhang, Ying, S. J., An, W. J., Lian, H., Zhou, G. B., and Han, Z. Y., “Effects of dietary betaine supplementation subjected to heat stress on milk performances and physiology indices in dairy cow”, vol. 13, pp. 7577-7586, 2014.

This study aimed to determine whether feeding betaine to cows elevates their production performance during summer heat stress. Thirty-two lactating Holstein cows were randomly divided into 4 groups: the control group, which received a total mixed ration (TMR), and 3 experimental groups that received TMR blended with 10 g/day (group I), 15 g/day (group II), and 20 g/day (group III) betaine for 8 weeks. Milk and blood were sampled throughout the experimental period. The average maximum and minimum air temperatures were 28.3 and 24.1°C, respectively.

Acute heat stress and dietary methionine effects on IGF-I, GHR, and UCP mRNA expression in liver and muscle of quails

A. P. Del Vesco, Gasparino, E., Zancanela, V., Grieser, D. O., Guimarães, S. E. F., Nascimento, C. S., Voltolini, D. M., Constantin, J., and Gasparin, F. S., “Acute heat stress and dietary methionine effects on IGF-I, GHR, and UCP mRNA expression in liver and muscle of quails”, vol. 13, pp. 7294-7303, 2014.

This study evaluated the expression of insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), and uncoupling protein (UCP) mRNA in muscle and liver of quails that were in thermal comfort or exposed to heat stress and that were fed diets with or without methionine supplementation. Meat quails were fed a diet that either met the nutritional demands for methionine (MS) or did not meet this demand (methionine-deficient diet, MD). The animals were either kept at a thermal comfort temperature (25°C) or exposed to heat stress (38°C for 24 h starting on the 6th day).

Association of Hsp60 expression with damage to rat myocardial cells exposed to heat stress in vivo and in vitro

Y. F. Cheng, Sun, J. R., Chen, H. B., Abdelnasir, A., Tang, S., Kemper, N., Hartung, J., and Bao, E. D., “Association of Hsp60 expression with damage to rat myocardial cells exposed to heat stress in vivo and in vitro”, vol. 13, pp. 9371-9381, 2014.

To investigate the protective role of Hsp60 against stress damage and its role in the sudden death of stressed animals, changes in the levels of Hsp60 protein and hsp60 mRNA of myocardial cells in vivo and in vitro were studied. In addition, the relationship between Hsp60 expression and heat-induced damage was also studied. Rats were exposed to a temperature of 42° ± 1°C for 0, 20, 40, 60, 80, or 100 min. More than 50% of the rats died suddenly within 100 min.

Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo

A. Abdelnasir, Sun, J. R., Cheng, Y. F., Chen, H. B., Tang, S., Kemper, N., Hartung, J., and Bao, E. D., “Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo”, vol. 13, pp. 10787-10802, 2014.

The aim of the present study was to identify the correlation between expression of heat shock protein 47 (Hsp47) and stress injury in heat-stressed myocardial cells and to compare variations in Hsp47 expression in rat myocardial cells exposed to different heat stress for varying periods in vitro and in vivo. Exposure to heat stress at 42°C resulted in similar induction patterns of the heart damage-related enzyme aspartate aminotransferase in the supernatants of H9c2 cells and in the serum of rats.

Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro

Z. J. Liu, Lv, Y. J., Zhang, M., Yue, Z. H., Tang, S., Islam, A., Rehana, B., Bao, E. D., and Hartung, J., “Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro”, vol. 11, pp. 4728-4738, 2012.

We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases in aspartate transaminase, lactate dehydrogenase, and creatine kinase enzymatic activities in the myocardial cell culture media were observed during heat stress, suggesting that the integrity of the myocardial cells was altered.

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