Temporal variations of Hsp60 and HSF-1 in primary rat myocardial cells in vitro under heat stress
“Temporal variations of Hsp60 and HSF-1 in primary rat myocardial cells in vitro under heat stress”, vol. 12, pp. 3003-3016, 2013.
, The mechanisms involved in sudden animal death due to acute heart failure during heat stress are not well understood. We examined the relationship between heat stress-induced variations of protective Hsp60 and expression of its regulatory factor, HSF-1, in heat-stressed primary myocardial cells of neonatal rats in vitro through cardiac enzyme detection, immunoblotting, immunocytochemistry, and qPCR. Increases in cardiac damage-related enzyme levels demonstrated injury to myocardial cells after heat exposure at 42°C.