Green fluorescent protein

Agrobacterium-mediated transformation of Fusarium proliferatum

J. Bernardi-Wenzel, Quecine, M. C., Azevedo, J. L., Pamphile, J. A., Bernardi-Wenzel, J., Quecine, M. C., Azevedo, J. L., and Pamphile, J. A., “Agrobacterium-mediated transformation of Fusarium proliferatum”, vol. 15, p. -, 2016.

Fusarium proliferatum is an important pathogen that is associated with plant diseases and primarily affects aerial plant parts by producing different mycotoxins, which are toxic to humans and animals. Within the last decade, this fungus has also been described as one of the causes of red root rot or sudden death syndrome in soybean, which causes extensive damage to this crop. This study describes the Agrobacterium tumefaciens-mediated transformation of F. proliferatum as a tool for the disruption of pathogenicity genes.

Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein

Q. J. Xiang, Zhai, J. F., Zhang, M., and Zhang, B., “Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein”, vol. 14, pp. 17834-17846, 2015.

In this study, the in vivo interaction system of oligopeptide permease (Opp) proteins was analyzed, and a high expression system of inner membrane protein OppC was constructed by flexible usage of the green fluorescent protein (GFP). The Escherichia coli OppC gene, which encodes a transmembrane component of oligopeptide transporter, was cloned into different vectors. Recombinant plasmids were transformed into different E. coli strains, and the expression conditions were optimized.

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