Grass carp hemorrhagic disease

Cloning, characterization and expression analysis of coagulation factor II gene in grass carp (Ctenopharyngodon idella)

B. H. Xu, Chen, K. J., Yao, Y. B., Liu, Q. L., Xiao, T. Y., Su, J. M., and Peng, H. Z., Cloning, characterization and expression analysis of coagulation factor II gene in grass carp (Ctenopharyngodon idella), vol. 14, pp. 13764-13778, 2015.

Here, we characterized the structure and function of the coagulation factor II (FII) gene in grass carp and determined its role in coagulation mechanisms. The FII gene EST was obtained using a constructed splenic transcriptome database; the full-length FII gene sequence was obtained by 3' and 5' RACE. The open reading frame (ORF) of FII was cloned and the full-length gene was found to be 1718 bp, with an ORF of 1572 bp; the gene contained a 25 bp 5'-untranslated region (UTR) and 108 bp 3'-UTR.

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