Granulosa cells

miR-34a targets the inhibin beta B gene, promoting granulosa cell apoptosis in the porcine ovary

F. Tu, Pan, Z. X., Yao, Y., Liu, H. L., Liu, S. R., Xie, Z., and Li, Q. F., miR-34a targets the inhibin beta B gene, promoting granulosa cell apoptosis in the porcine ovary, vol. 13, pp. 2504-2512, 2014.

During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in pig ovarian follicles. In addition, inhibin beta B was found to be a miR-34a target gene, based on luciferase reporter assays, quantitative RT-PCR and Western blotting.

Rescue from dominant follicle atresia by follicle-stimulating hormone in mice

X. L. Zhou, Teng, Y., Cao, R., Fu, H., Xiong, K., Sun, W. X., Zhu, C. C., Huang, X. J., Xiao, P., and Liu, H. L., Rescue from dominant follicle atresia by follicle-stimulating hormone in mice, vol. 12, pp. 2945-2952, 2013.

We investigated the effects of follicle-stimulating hormone (FSH) on atresia of the dominant follicle and changes in relevant apoptosis genes in granulosa cells of dominant follicles regulated by FSH in vivo. Four-week-old mice were administered FSH by intraperitoneal injection to induce follicular maturation. Granulosa cells of dominant follicles were collected at 48, 72, and 96 h after the first FSH injection. Phosphate-buffered saline was injected as a control.

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