Gramineae plants

Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses (Poaceae)

L. Yu, Wu, X., Tang, X., and Yan, B., Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses (Poaceae), vol. 9. pp. 1321-1325, 2010.

cDNA fragments of lea3 genes with a high GC content (from 68 to 77%) were found in several Poaceae, including Sorghum vulgare, Saccharum officinarum, Oryza officinalis, Oryza meyeriana, Ampelocalamus calcareus, Cynodon dactylon, and Zizania latifoli. They were successfully isolated by means of optimal experimental parameters, which included dimethyl sulfoxide as additive and degenerate primers “AGETKAS” and “AGKDKTG”, and their sequences were analyzed.

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