Goat

Transcript analysis of a goat mesenteric lymph node by deep next-generation sequencing

G. X. E, Zhao, Y. J., Na, R. S., Huang, Y. F., E, G. X., Zhao, Y. J., Na, R. S., and Huang, Y. F., “Transcript analysis of a goat mesenteric lymph node by deep next-generation sequencing”, vol. 15, p. -, 2016.

Deep RNA sequencing (RNA-seq) provides a practical and inexpensive alternative for exploring genomic data in non-model organisms. The functional annotation of non-model mammalian genomes, such as that of goats, is still poor compared to that of humans and mice. In the current study, we performed a whole transcriptome analysis of an intestinal mucous membrane lymph node to comprehensively characterize the transcript catalogue of this tissue in a goat. Using an Illumina HiSeq 4000 sequencing platform, 9.692 GB of raw reads were acquired.

Analysis of POU1F1 gene DdeI polymorphism in Chinese goats

M. J. Li, Zhang, C. M., Lan, X. Y., Fang, X. T., Lei, C. Z., Chen, H., Li, M. J., Zhang, C. M., Lan, X. Y., Fang, X. T., Lei, C. Z., and Chen, H., “Analysis of POU1F1 gene DdeI polymorphism in Chinese goats”, vol. 15, p. -, 2016.

As a member of the POU-domain family, the POU1F1 is a positive regulator for growth hormone, prolactin and thyroid-stimulating hormone b, by binding to target DNA promoters as a dimer in mammals. This study described the polymorphisms at the goat POU1F1-DdeI locus and analyzed the distribution of alleles in 15 indigenous Chinese goat breeds. The PCR-RFLP analysis showed a predominance of the D1D1 genotype and the D1 allele, with average frequencies of 0.550 and 0.790, respectively, irrespective of goat utility type.

Subscribe to Goat