Glycine max

Cloning and functional prediction of differentially expressed genes in the leaves of Glycine max parents and hybrids at the seedling stage

J. Zhang, Yao, D., Wang, P., Guan, S. Y., Ma, J., and Fu, Y. P., Cloning and functional prediction of differentially expressed genes in the leaves of Glycine max parents and hybrids at the seedling stage, vol. 13, pp. 5474-5483, 2014.

Here, we compare the molecular mechanism of soybean heterosis through the differential expression of basic cloning. Specifically, we cloned 22 differentially expressed cDNA fragments from hybrid combinations of Jilin 38 x EXP (which had obvious yield advantages) and their parents. In addition, we compared the homology of these fragments and predicted their functions.

Prediction of potential novel microRNAs in soybean when in symbiosis

G. A. Barros-Carvalho, Paschoal, A. R., Marcelino-Guimarães, F. C., and Hungria, M., Prediction of potential novel microRNAs in soybean when in symbiosis, vol. 13, pp. 8519-8529, 2014.

MicroRNAs (miRNAs) are small molecules, noncoding proteins that are involved in many biological processes, especially in plants; among these processes is nodulation in the legume. Biological nitrogen fixation is a key process, with critical importance to the soybean crop. This study aimed to identify the potential of novel miRNAs to act during the root nodulation process.

Construction of a molecular database for soybean cultivar identification in Brazil

M. B. Oliveira, Vieira, E. S. N., and Schuster, I., Construction of a molecular database for soybean cultivar identification in Brazil, vol. 9, pp. 705-720, 2010.

The narrow genetic base of soybean makes cultivar characterization based on morphological descriptors difficult; this characterization is mainly done for registration and protection. Correct characterization of cultivars could be achieved through molecular markers, since the frequencies of each allele in the population are known. Consequently, we developed a molecular characterization method and initiated the construction of a molecular database for soybean cultivar identification. Thirty-two soybean cultivars were analyzed with 48 fluorescent-labeled microsatellite markers.

Cloning and quantitative expression analysis of drought-induced genes in soybean

R. Stolf-Moreira, Medri, M. E., Neumaier, N., Lemos, N. G., Brogin, R. L., Marcelino, F. C., de Oliveira, M. C. N., Farias, J. R. B., Abdelnoor, R. V., and Nepomuceno, A. L., Cloning and quantitative expression analysis of drought-induced genes in soybean, vol. 9, pp. 858-867, 2010.

We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and drought-sensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought conditions. Sequences of Arabidopsis thaliana DREB-family proteins were used to build a phylogenetic tree through the alignment of the conserved regions near the AP2 domain.

Soybean physiology and gene expression during drought

R. Stolf-Moreira, Medri, M. E., Neumaier, N., Lemos, N. G., Pimenta, J. A., Tobita, S., Brogin, R. L., Marcelino-Guimarães, F. C., Oliveira, M. C. N., Farias, J. R. B., Abdelnoor, R. V., and Nepomuceno, A. L., Soybean physiology and gene expression during drought, vol. 9, pp. 1946-1956, 2010.

Soybean genotypes MG/BR46 (Conquista) and BR16, drought-tolerant and -sensitive, respectively, were compared in terms of morphophysiological and gene-expression responses to water stress during two stages of development. Gene-expression analysis showed differential responses in Gmdreb1a and Gmpip1b mRNA expression within 30 days of water-deficit initiation in MG/BR46 (Conquista) plants. Within 45 days of initiating stress, Gmp5cs and Gmpip1b had relatively higher expression.

Soybeans as bioreactors for biopharmaceuticals and industrial proteins

G. R. Vianna, Cunha, N. B., Murad, A. M., and Rech, E. L., Soybeans as bioreactors for biopharmaceuticals and industrial proteins, vol. 10. pp. 1733-1752, 2011.

Plants present various advantages for the production of biomolecules, including low risk of contamination with prions, viruses and other pathogens, scalability, low production costs, and available agronomical systems. Plants are also versatile vehicles for the production of recombinant molecules because they allow protein expression in various organs, such as tubers and seeds, which naturally accumulate large amounts of protein. Among crop plants, soybean is an excellent protein producer.

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