Glutathione-S-transferase

Effect of the ARG1 gene on arsenic resistance of 293T cells

X. L. Mu, Li, L., and Li, H., Effect of the ARG1 gene on arsenic resistance of 293T cells, vol. 12, pp. 6825-6837, 2013.

To study the relationship between arsenic resistance of 293T cells and overexpression of ARG1, the ARG1 gene in a recombinant plasmid was transfected into 293T cells via liposomes, and then ARG1 overexpression was examined by real-time PCR and immunocytochemistry. The survival rate, arsenic accumulation and arsenic efflux, GSH level, and GST activity of 293T cells overexpressing ARG1 were assayed by MTT, atomic absorption spectrophotometry, and DTNB, and expression of MRP-2 was detected by Western blot analysis.

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