Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers
“Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers”, vol. 13, pp. 4361-4371, 2014.
, Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella with the linear 1Dx5 gene without selectable markers. The highest transient β-glucuronidase expression was obtained when scuttles were bombarded at 1100 psi with a 9-cm target distance.