β-glucuronidase (GUS)

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers

J. B. Qin, Wang, Y., and Zhu, C. Q., Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers, vol. 13, pp. 4361-4371, 2014.

Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella with the linear 1Dx5 gene without selectable markers. The highest transient β-glucuronidase expression was obtained when scuttles were bombarded at 1100 psi with a 9-cm target distance.

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