Germplasm conservation

Development and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria leucospilota

G. Dai, Li, Z. B., Shangguan, J. B., Ning, Y. F., Deng, H. W., Yuan, Y., Huang, Y. S., Yang, H., and Lu, J., Development and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria leucospilota, vol. 14, pp. 538-541, 2015.

Holothuria leucospilota is a tropical holothurian species that is widely distributed in the tropical and sub-tropical India-Western Pacific Region. Eight polymorphic microsatellite loci were developed from H. leucospilota by using the protocol fast isolation by amplified fragment length polymorphism of sequences containing repeats and tested in 30 individuals from Hainan Island in China. The number of alleles was 2-6 and polymorphism information content ranged from 0.371-0.694.

Genetic diversity of worldwide Jerusalem artichoke (Helianthus tuberosus) germplasm as revealed by RAPD markers

P. P. Wangsomnuk, Khampa, S., Wangsomnuk, P., Jogloy, S., Mornkham, T., Ruttawat, B., Patanothai, A., and Fu, Y. B., Genetic diversity of worldwide Jerusalem artichoke (Helianthus tuberosus) germplasm as revealed by RAPD markers, vol. 10, pp. 4012-4025, 2011.

Jerusalem artichoke (Helianthus tuberosus) is a wild relative of the cultivated sunflower (H. annuus); it is an old tuber crop that has recently received renewed interest. We used RAPD markers to characterize 147 Jerusalem artichoke accessions from nine countries. Thirty RAPD primers were screened; 13 of them detected 357 reproducible RAPD bands, of which 337 were polymorphic. Various diversity analyses revealed several different patterns of RAPD variation. More than 93% of the RAPD variation was found within accessions of a country.

Estimation of genetic distance between 10 maize accessions with varying response to different levels of soil moisture

M. Aslam, Awan, F. S., Khan, I. A., and Khan, A. I., Estimation of genetic distance between 10 maize accessions with varying response to different levels of soil moisture, vol. 8, pp. 1459-1465, 2009.

Ten maize accessions (NC-9, A50-2, M-14, B-42, NC-3, T-7, N-48-1, B-34, USSR, and WFTMS) were studied to estimate the genetic distance on molecular level by random amplified polymorphic DNA. These accessions were selected on the basis of their variable responses against different levels of moisture. Twenty-five primers were used to test genetic diversity, of which 14 were observed to be polymorphic. Ninety-three loci were amplified; among these, 77 showed polymorphism and the other 16 were monomorphic.

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