Germination

Effect of ethyl methyl sulfonate concentration and different treatment conditions on germination and seedling growth of the cucumber cultivar Chinese long (9930)

S. N. M. Shah, Gong, Z. - H., Arisha, M. H., Khan, A., and Tian, S. - L., Effect of ethyl methyl sulfonate concentration and different treatment conditions on germination and seedling growth of the cucumber cultivar Chinese long (9930), vol. 14, pp. 2440-2449, 2015.

We attempted to create a new germplasm of cucumber cultivar Chinese long (9930) using different doses of ethyl methyl sulfonate (EMS) to induce variability. We tested EMS concentration (0, 0.5, 1.0, 1.5, 2, 3% v/v) with post-treatment (0.1 M Na2S2O3 and water), EMS concentration (0, 0.5, 1.0, 1.5% v/v) over different treatment times (8, 16, 24 h), and EMS concentration (0, 0.5, 1.0, 1.5% v/v) with different treatment temperatures (20 and 28°C).

Physiological quality and gene expression during the development of habanero pepper (Capsicum chinense Jacquin) seeds

H. O. Santos, Von Pinho, E. V. R., Von Pinho, I. V., Dutra, S. M. F., Andrade, T., and Guimarães, R. M., Physiological quality and gene expression during the development of habanero pepper (Capsicum chinense Jacquin) seeds, vol. 14, pp. 5085-5098, 2015.

Phytohormones have different characteristics and functions, and they may be subject to changes in their gene expression and synthesis during seed development. In this study, we evaluated the physiological qualities of habanero peppers (Capsicum chinense Jacquin) during seed development and the expression of genes involved in germination. Seeds were obtained from fruits harvested at different stages of development [i.e., 14, 21, 28, 35, 42, 49, 56, 63, and 70 days after anthesis (DAA)].

A non-destructive genotyping system from a single seed for marker-assisted selection in watermelon

G. Meru, McDowell, D., Waters, V., Seibel, A., Davis, J., and McGregor, C., A non-destructive genotyping system from a single seed for marker-assisted selection in watermelon, vol. 12, pp. 702-709, 2013.

Genomic tools for watermelon breeding are becoming increasingly available. A high throughput genotyping system would facilitate the use of DNA markers in marker-assisted selection. DNA extraction from leaf material requires prior seed germination and is often time-consuming and cost prohibitive. In an effort to develop a more efficient system, watermelon seeds of several genotypes and various seed sizes were sampled by removing ⅓ or ½ sections from the distal ends for DNA extraction, while germinating the remaining proximal parts of the seed.

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