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In-depth analysis of internal control genes for quantitative real-time PCR in Brassica oleracea var. botrytis

X. G. Sheng, Zhao, Z. Q., Yu, H. F., Wang, J. S., Zheng, C. F., Gu, H. H., Sheng, X. G., Zhao, Z. Q., Yu, H. F., Wang, J. S., Zheng, C. F., and Gu, H. H., In-depth analysis of internal control genes for quantitative real-time PCR in Brassica oleracea var. botrytis, vol. 15, p. -, 2016.

Quantitative reverse-transcription PCR (qRT-PCR) is a versatile technique for the analysis of gene expression. The selection of stable reference genes is essential for the application of this technique. Cauliflower (Brassica oleracea L. var. botrytis) is a commonly consumed vegetable that is rich in vitamin, calcium, and iron. Thus far, to our knowledge, there have been no reports on the validation of suitable reference genes for the data normalization of qRT-PCR in cauliflower.

Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head

X. N. Wang, Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., Wang, J. C., Wang, X. N., Yang, Q. W., Du, Z. W., Yu, T., Qin, Y. G., Song, Y., Xu, M., and Wang, J. C., Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head, vol. 15, p. -, 2016.

This study aimed to evaluate 12 genes (18S, GAPDH, B2M, ACTB, ALAS1, GUSB, HPRT1, PBGD, PPIA, PUM1, RPL29, and TBP) for their reliability and stability as reference sequences for real-time quantitative PCR (RT-qPCR) in bone marrow-derived mesenchymal stem cells (BMSCs) isolated from patients with avascular necrosis of the femoral head (ANFH). BMSCs were isolated from 20 ANFH patients divided into four groups according to etiology, and four donors with femoral neck fractures.

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