Genomic library

Isolation and characterization of microsatellite DNA in the piracema fish Prochilodus lineatus(Characiformes)

G. M. Yazbeck and Kalapothakis, E., Isolation and characterization of microsatellite DNA in the piracema fish Prochilodus lineatus(Characiformes), vol. 6, pp. 1026-1034, 2007.

We described five novel microsatellite loci for the piracema fish species Prochilodus lineatus (Characiformes), endemic to South America and of extreme importance to both commercial and artisanal fisheries across its occurrence area. A primary, unenriched genomic library was constructed and radioactively screened for repetitive motifs. Positive clones were automatically sequenced and based on the design of new primers, polymerase chain reaction assays were carried out to determine optimum reaction and electrophoretic conditions for each characterized locus.

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