Genomic DNA extraction

A rapid and inexpensive method for isolation of total DNA from Trichoderma spp (Hypocreaceae)

J. C. Vazquez-Angulo, Mendez-Trujillo, V., González-Mendoza, D., Morales-Trejo, A., Grimaldo-Juarez, O., and Cervantes-Díaz, L., A rapid and inexpensive method for isolation of total DNA from Trichoderma spp (Hypocreaceae), vol. 11. pp. 1379-1384, 2012.

Extraction of high-quality genomic DNA for PCR amplification from filamentous fungi is difficult because of the complex cell wall and the high concentrations of polysaccharides and other secondary metabolites that bind to or co-precipitate with nucleic acids. We developed a modified sodium dodecyl sulfate/phenol protocol, without maceration in liquid nitrogen and without a final ethanol precipitation step. The A260/280 absorbance ratios of isolated DNA were approximately 1.7-1.9, demonstrating that the DNA fraction is pure and can be used for analysis.

Fast protocol for extraction of DNA from Prosopis spp leaves (plant adapted to arid environment) without liquid nitrogen

C. Y. Michel-López, González-Mendoza, D., and Grimaldo-Juarez, O., Fast protocol for extraction of DNA from Prosopis spp leaves (plant adapted to arid environment) without liquid nitrogen, vol. 12, pp. 4090-4094, 2013.

The extraction of high-quality genomic DNA from Prosopis spp for polymerase chain reaction (PCR) amplification is complicated, owing to the presence of a high percentage of secondary metabolites that bind to or co-precipitate with nucleic acids. In the present study, we report a modified sodium dodecyl sulfate/phenol protocol that eliminates the use of liquid nitrogen in the maceration process, β-mercaptoethanol in the buffer extraction, and the ethanol precipitation step.

A high-throughput, high-quality plant genomic DNA extraction protocol

H. Li, Li, J., Cong, X. H., Duan, Y. B., Li, L., Wei, P. C., Lu, X. Z., and Yang, J. B., A high-throughput, high-quality plant genomic DNA extraction protocol, vol. 12, pp. 4526-4539, 2013.

The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block, our protocol provides high throughput. Without the need for phenol-chloroform and liquid nitrogen or dry ice, our protocol is safer and more cost-efficient than traditional DNA extraction methods.

A rapid method for isolation of total DNA from pathogenic filamentous plant fungi

D. González-Mendoza, Argumedo-Delira, R., Morales-Trejo, A., Pulido-Herrera, A., Cervantes-Díaz, L., Grimaldo-Juarez, O., and Alarcón, A., A rapid method for isolation of total DNA from pathogenic filamentous plant fungi, vol. 9, pp. 162-166, 2010.

DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terrestris, which causes pink root in onions.

Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa

S. C. O. Melo, Pungartnik, C., Cascardo, J. C. M., and Brendel, M., Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa, vol. 5, pp. 851-855, 2006.

DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads.

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