Genetic variability

Differential responses of one hundred tomato genotypes grown under cadmium stress

M. M. Hussain, Saeed, A., Khan, A. A., Javid, S., and Fatima, B., Differential responses of one hundred tomato genotypes grown under cadmium stress, vol. 14, pp. 13162-13171, 2015.

Due to increased global concern over the deleterious effects of toxic heavy metals in the environment, it has become necessary to develop plant genotypes that limit the uptake of heavy metals to aerial edible parts. To address this concern, we performed a glasshouse experiment to assess variations within tomato germplasm for cadmium (Cd) tolerance under control conditions and under simulated stress conditions. Significant differences (P

Comparison of gSSR and EST-SSR markers for analyzing genetic variability among tomato cultivars (Solanum lycopersicum L.)

R. Zhou, Wu, Z., Jiang, F. L., and Liang, M., Comparison of gSSR and EST-SSR markers for analyzing genetic variability among tomato cultivars (Solanum lycopersicum L.), vol. 14, pp. 13184-13194, 2015.

In order to study genetic variability and develop better strategies for the utilization of 48 tomato cultivars from America, China, the Netherlands, and Portugal, genomic simple sequence repeat (gSSR) and EST-derived SSR (EST-SSR) markers were applied. In all, 15 of 82 gSSR and 18 of 115 EST-SSR markers showed polymorphic loci. There were 995 and 2072 clear fragments amplified by polymorphic gSSR and EST-SSR markers, respectively. The total and average number of alleles detected by EST-SSRs (75, 4.2) was more than gSSRs (54, 3.6) as a result of some multi-locus EST-SSRs.

High genetic variability and polychromatism in Pachycoris torridus (Heteroptera: Scutelleridae)

T. S. Souza-Firmino, Alevi, K. C. C., Pereira, L. L. V., Souza, E. R. S., Júnior, F. C. S., Banho, C. A., Carmo, G. O., and Itoyama, M. M., High genetic variability and polychromatism in Pachycoris torridus (Heteroptera: Scutelleridae), vol. 14, pp. 14300-14307, 2015.

The stink bug Pachycoris torridus is listed among the most polyphagous insects in the world and it is a major pest of diverse crops, in particular the physic nut Jatropha curcas, which is used as a raw material for biodiesel production. A peculiar characteristic of this species is its high phenotypic variability, a characteristic that makes identification difficult: P. torridus has been described as a new species eight times. Thus, to aid in identification, genetic characterization of this insect was performed.

Genetic dissimilarity and selection of putative mutants of Terra Maranhão plantain cultivar using the Ward-MLM strategy

R. V. Reis, Amorim, E. P., Amorim, V. B. O., Ferreira, C. F., Pestana, R. K. N., Ledo, C. A. S., Gonçalves, Z., and Borém, A., Genetic dissimilarity and selection of putative mutants of Terra Maranhão plantain cultivar using the Ward-MLM strategy, vol. 14, pp. 15339-15348, 2015.

The objective of this study was to evaluate genetic variability and select putative mutants of Terra Maranhão plantain cultivar (AAB genome) subjected to gamma radiation based on agronomic data and inter simple sequence repeat molecular marker profiles using the Ward-MLM strategy. A total of 233 irradiated plants and 41 controls were assessed. The agronomic and molecular data were subjected to the Ward-MLM statistical algorithm in the SAS program.

Population genetic structure in Phyla scaberrima from Mexico and Colombia assessed by AFLP markers and implications for conservation

L. G. Androcioli, Ruas, E. A., Rodrigues, L. A., Ruas, C. F., Perilla, H. E. R., and Ruas, P. M., Population genetic structure in Phyla scaberrima from Mexico and Colombia assessed by AFLP markers and implications for conservation, vol. 14, pp. 15697-15704, 2015.

Phyla scaberrima (Verbenaceae) is a herbaceous peren­nial species that is distributed from Mexico (center of origin) to Colom­bia, growing in forest and swamp edges or grasslands from sea level up to an altitude of 1800 m. The chemical properties and uses in popular medicine have drastically affected the population size of this species. In this study, we investigated genetic variability in populations of P. scaberrima using AFLP markers.

Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers

C. A. Nogueira, Stafuzza, N. B., Ribeiro, T. P., Prado, A. D. L., Menezes, I. P. P., Peixoto, N., Gonçalves, P. J., and Almeida, L. M., Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers, vol. 14, pp. 15996-16005, 2015.

Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six botanic varieties that differ morphologically mainly in the shapes of their leaves and flowers.

Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil

R. R. Simplicio, Waldschmidt, A. M., Amorim, M. B., Almeida, B. S., and Pereira, D. G., Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil, vol. 14, pp. 18241-18248, 2015.

Maytenus truncata (Celastraceae) is a plant species widely used in the treatment of ulcers and tumors. Despite the intensive harvest of native specimens in the State of Bahia, northeastern Brazil, there is no information about the genetic variability or structure of this species. Therefore, the goal of this study was to estimate the genetic diversity and population structure of M. truncata based on inter simple sequence repeat (ISSR) molecular markers.

Comparison of eight methods of genomic DNA extraction from babassu

J. P. G. Viana, Borges, A. N. C., Lopes, A. C. A., Gomes, R. L. F., Britto, F. B., Lima, P. S. C., and Valente, S. E. S., Comparison of eight methods of genomic DNA extraction from babassu, vol. 14, pp. 18003-18008, 2015.

Babassu (Orbignya phalerata Martius) is one of the most important palms in Brazil because of the largest morphological variation, wide geographic distribution, and high socio-economic importance. The diversity present in babassu germplasm should be protected against loss to ensure their use with high productivity. Study of the available variability in populations of babassu is necessary to develop conservation strategies. The study of genetic variability can be conducted using molecular markers and many of these studies require significant quantity of high-quality DNA.

Population structure and genetic diversity in Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae): is there a correlation between genetic and geographic proximity?

A. L. S. Mascarenhas, Waldschmidt, A. M., and Silva, Jr., J. C., Population structure and genetic diversity in Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae): is there a correlation between genetic and geographic proximity?, vol. 14, pp. 9793-9803, 2015.

Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae) is a minuscule insect species, which forms galls, is subsocial, and parthenogenetic. It is associated with Ficus benjamina L. (Moraceae) and has a pantropical occurrence. The paucity of genetic studies on the order Thysanoptera led us to use inter-simple sequence repeat molecular marker to assess intra- and inter-gall, as well as intra- and inter-site, genetic variability and population structure of G. uzeli.

Genetic structure of natural populations of Theobroma in the Juruena National Park, Mato Grosso State, Brazil

B. M. Silva, Rossi, A. A. B., Dardengo, J. F. E., Silva, C. R., Silva, I. V., Silva, M. L., and Silva, C. J., Genetic structure of natural populations of Theobroma in the Juruena National Park, Mato Grosso State, Brazil, vol. 14, pp. 10365-10375, 2015.

Several species within the genus Theobroma have particularly high economic value, including T. cacao and T. grandiflorum. Other species in this genus, such as T. speciosum and T. subincanum, have potential value for use in the conservation of genetic diversity in breeding programs. These latter species could also be domesticated or improved to produce commercial products. Using 13 simple sequence repeat loci, the population structure and genetic diversity of T. speciosum and T.

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