Genetic structure

Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China

Y. - W. Yang, Yang, Z. - Y., Yan, M. - R., Qian, Z. - G., and Guan, K. - Y., Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China, vol. 13, pp. 8220-8224, 2014.

Twenty-four microsatellite markers were isolated from the genomic DNA of Amomum tsaoko Crevost et Lemaire, an important economic plant in China, using the method fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 60 individuals from three populations in Yunnan Province, China, and nine of them were polymorphic. The number of alleles per polymorphic locus was 2, and the expected and observed heterozygosities ranged from 0.224 to 0.513, and from 0.050 to 0.600, respectively.

Nineteen polymorphic microsatellite markers developed for Trachinotus ovatus

Z. Z. Xie, Huang, M. W., Xu, W., Peng, C., He, J. N., Meng, Z. N., Zhang, Y., Li, S. S., and Lin, H. R., Nineteen polymorphic microsatellite markers developed for Trachinotus ovatus, vol. 13, pp. 10518-10522, 2014.

To evaluate the population genetic diversity of the ovate pompano, we isolated and characterized 19 microsatellite markers using a (CA)13-enriched genomic library. Polymorphism was assessed in 30 individuals from a single population collected from the Daya Bay Aquaculture Center, Guangdong, China. The number of alleles per locus ranged from 2 to 18 with an average of 7.8. The observed and expected heterozygosities varied from 0.2667 to 1.000 and from 0.3960 to 0.9435, respectively.

Seventeen polymorphic microsatellite markers developed for the Javelin goby, Synechogobius hasta (Gobiidae)

C. - C. Bai, Liu, S. - F., Zhuang, Z. - M., Yuan, Y. - J., Liu, H. - B., and Dai, F. - Q., Seventeen polymorphic microsatellite markers developed for the Javelin goby, Synechogobius hasta (Gobiidae), vol. 11. pp. 1465-1468, 2012.

Synechogobius hasta is an important commercial marine fish with distinctive features of rapid growth and short lifespan. We isolated and characterized 17 microsatellite markers for S. hasta using a (GT)13-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the northern coastal waters of the Yellow Sea. The number of alleles per locus ranged from 2 to 23, with a mean of 11.3.

Genetic diversity of Setipinna taty (Engraulidae) populations from the China Sea based on mitochondrial DNA control region sequences

H. Y. Li, Xu, T. J., Cheng, Y. Z., Sun, D. Q., and Wang, R. X., Genetic diversity of Setipinna taty (Engraulidae) populations from the China Sea based on mitochondrial DNA control region sequences, vol. 11, pp. 1230-1237, 2012.

The genetic diversity of Setipinna taty, which is commercially fished in the China Sea, was studied based on mitochondrial DNA control region sequences. PCR was used to amplify the control region fragment in 100 individuals of S. taty collected from Weihai (WH), Yantai (YT), Zhoushan (ZS), Xiangshan (XS), and Ninghai (NH) in China. A control region fragment of 656 bp was successfully sequenced in these 100 individuals. The A+T content of this S. taty control region was 71.7%; 172 variable sites and 62 haplotypes were found.

SRAP analysis of genetic diversity of nine native populations of wild sugarcane, Saccharum spontaneum, from Sichuan, China

D. Chang, Yang, F. Y., Yan, J. J., Wu, Y. Q., Bai, S. Q., Liang, X. Z., Zhang, Y. W., and Gan, Y. M., SRAP analysis of genetic diversity of nine native populations of wild sugarcane, Saccharum spontaneum, from Sichuan, China, vol. 11, pp. 1245-1253, 2012.

Saccharum spontaneum is a wild sugarcane species that is native to and widely distributed in China. It has been extensively used in sugarcane breeding programs, and is being tested for the development of bioenergy cultivars. In order to provide basic information for the exploitation of this species, we analyzed genetic variation among and within native S. spontaneum populations collected from Sichuan, China. Eighty plants from nine native populations were sampled.

Genetic characterization of Red Junglefowl (Gallus gallus), Thai indigenous chicken (Gallus domesticus), and two commercial lines using selective functional genes compared to microsatellite markers

P. Akaboot, Duangjinda, M., Phasuk, Y., Kaenchan, C., and Chinchiyanond, W., Genetic characterization of Red Junglefowl (Gallus gallus), Thai indigenous chicken (Gallus domesticus), and two commercial lines using selective functional genes compared to microsatellite markers, vol. 11, pp. 1881-1890, 2012.

Genetic characterization among Red Junglefowl (GS, Gallus gallus spadiceus), Thai indigenous chicken (TIC, Gallus domesticus) and commercial lines has been widely used for studies of genealogical origin, genetic diversity, and effects of selection. We compared the efficiency of genetic characterization of chicken populations that had been under different intensities of selection using selective functional gene versus microsatellite marker analyses.

Development and characterization of nine polymorphic microsatellites for the small brown planthopper Laodelphax striatellus (Hemiptera: Delphacidae)

J. - T. Sun, Li, J. - B., Yang, X. - M., and Hong, X. - Y., Development and characterization of nine polymorphic microsatellites for the small brown planthopper Laodelphax striatellus (Hemiptera: Delphacidae), vol. 11. pp. 1526-1531, 2012.

Nine microsatellite loci were isolated from the genome of Laodelphax striatellus (Homoptera: Delphacidae) by constructing (TC)6(AC)5 and (AG)6(AC)5 compound SSR-enriched libraries using suppression-PCR procedures. These loci were found to be highly polymorphic, with 13 to 30 alleles per locus in the three populations that we investigated (Jiangsu, Shandong and Zhejiang). The observed and expected heterozygosities ranged from 0.255 to 0.833 and 0.392 to 0.929, respectively.

Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

H. S. An, Lee, J. W., and Park, J. Y., Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis, vol. 11, pp. 3904-3922, 2012.

Populations of the Pacific abalone, Haliotis discus hannai, have been severely overexploited over the past few decades in Korea. Information regarding the levels of genetic variability and structure within populations is insufficient for the development of effective strategies for conservation of genetic diversity of this species.

Isolation and characterization of 48 polymorphic microsatellite markers for the blood clam Scapharca broughtonii (Arcidae)

J. - T. Tian, Liu, Z. - H., Zhou, L. - Q., Wu, B., Liu, P., and Yang, A. - G., Isolation and characterization of 48 polymorphic microsatellite markers for the blood clam Scapharca broughtonii (Arcidae), vol. 11, pp. 4501-4507, 2012.

Blood clams (Scapharca broughtonii) are widely cultivated and consumed in noutheast Asia. Forty-eight polymorphic microsatellite loci were developed for this clam using magnetic-bead hybridization enrichment. The number of alleles per locus ranged from 2 to 14. Polymorphism of these loci was assessed in 30 individuals from a population collected from coastal areas of Qingdao, China.

Diversity and genetic structure among subpopulations of Gossypium mustelinum (Malvaceae)

M. F. Alves, Barroso, P. A. V., Ciampi, A. Y., Hoffmann, L. V., Azevedo, V. C. R., and Cavalcante, U., Diversity and genetic structure among subpopulations of Gossypium mustelinum (Malvaceae), vol. 12, pp. 597-609, 2013.

Gossypium mustelinum is the only cotton species native to Brazil; it is endemic to the semi-arid region of the northeast. The populations are found near perennial and semi-perennial sources of water, such as ponds or pools in intermittent streams. Problems with in situ conservation derive from human interference in its habitat, mainly because of excessive cattle grazing and deforestation. Establishing efficient strategies for in situ conservation requires knowledge of the genetic structure of the populations.

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