Genetic diversity

Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi

C. F. Sun, Ye, X., Tian, Y. Y., and Dong, J. J., Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi, vol. 14, pp. 9343-9352, 2015.

In order to provide genetic information for the selective breeding of Siniperca chuatsi, 14 microsatellite DNA loci were used to evaluate the genetic diversity and structure of four farmed populations and one wild population in China. The four cultivated populations were Foshan (FS), Jiangmen (JM), Nanjing (NJ), and Hongze Lake (HZL), and the wild population was collected from the Hubei HuangGang section of the Yangtze River (HG).

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers

M. P. Liu, Du, H. Y., Zhu, G. P., Fu, D. L., and Tana, W. Y., Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers, vol. 14, pp. 9722-9729, 2015.

Simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) markers were used to evaluate genetic diversity among 22 sweet kernel apricot accessions and 12 cultivars in China to provide information on how to improve the utilization of kernel apricot germplasms. The results showed that 10 pairs of SSR primers screened from 40 primer pairs amplified 43 allelic variants, all of which were polymorphic (100%), and 9 ISSR primers selected from 100 primers amplified 67 allelic variants with 50 polymorphic bands (74.63%).

Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm

R. S. Celestino, Zucchi, M. I., Pinheiro, J. B., Campos, J. B., Pereira, A. A., Bianchini, F. G., Lima, R. N., Arrigoni-Blank, M. F., Alves, P. B., and Blank, A. F., Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm, vol. 14, pp. 9452-9468, 2015.

Due to the economic interests in vetiver, Chrysopogon zizanioides (L.) Roberty, molecular and chemical studies are essential to generate information for its sustainable exploitation. The aim of this study was to undertake a molecular and chemical characterization of vetiver accessions of the active germplasm bank of the Universidade Federal de Sergipe.

Analysis of the genetic diversity of beach plums by simple sequence repeat markers

X. M. Wang, Wu, W. L., Zhang, C. H., Zhang, Y. P., Li, W. L., and Huang, T., Analysis of the genetic diversity of beach plums by simple sequence repeat markers, vol. 14, pp. 9693-9702, 2015.

The purpose of this study was to measure the genetic diversity of wild beach plum and cultivated species, and to determine the species relationships using SSRs markers. An analysis of genetic diversity from ten beach plum germplasms was carried out using 11 simple sequence repeat (SSR) primers selected from 35 primers to generate distinct PCR products. From this plant material, 44 allele variations were detected, with 3-5 alleles identified from each primer.

Genetic diversity of popcorn genotypes using molecular analysis

F. S. Resh, Scapim, C. A., Mangolin, C. A., Machado, M. F. P. S., Amaral, Jr., A. Tdo, Ramos, H. C. C., and Vivas, M., Genetic diversity of popcorn genotypes using molecular analysis, vol. 14, pp. 9829-9840, 2015.

In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were monomorphic and 92 were polymorphic. To calculate the genetic distances among the 26 genotypes, the complements of the Jaccard, Dice, and Rogers and Tanimoto similarity coefficients were used.

Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin

S. O. Bafeel, Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin, vol. 14, pp. 9974-9984, 2015.

The genetic diversity of Saudi locally growing sorghum (Sorghum bicolor) cultivars has not been thoroughly characterized. To understand the genomic patterns of diversification in Saudi sorghum cultivars (N = 7), random amplified polymorphic DNA (RAPD) was used as a rapid, inexpensive method for providing information regarding genomic variability below the species level. Six commercially available primers were initially used to select a single primer based on availability, universality, and its use with standard polymerase chain reaction (PCR) conditions.

Polymorphic microsatellite markers in the traditional Chinese medicinal plant Paris polyphylla var. yunnanensis

Y. Song, Li, M. F., Xu, J., Zhao, Z., and Chen, N. Z., Polymorphic microsatellite markers in the traditional Chinese medicinal plant Paris polyphylla var. yunnanensis, vol. 14, pp. 9939-9942, 2015.

Paris polyphylla var. yunnanensis is a traditional Chinese medicinal plant and is listed as vulnerable by the IUCN. This medicinal herb is well known for its analgesic and anti-inflammatory properties, most notably as an ingredient of the hemostatic compound “Yunnan Baiyao”. However, over-exploitation of the plant for economic purposes is pushing the species to the brink of extinction. Therefore, there is an urgent need to develop conservation strategies for this endangered species, for example, through assessment of its genetic structure and diversity.

Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment

M. Yildiz, Koçak, M., and Baloch, F. S., Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment, vol. 14, pp. 10588-10602, 2015.

Lack of requisite genetic variation in Turkish okra has necessitated the use of different types of markers for estimating the genetic diversity and identifying the source of variation. Transposable elements, present abundantly in plant genomes, generate genomic diversity through their replication and are thus an excellent source of molecular markers. We hypothesized that inter-primer binding site (iPBS)-retrotransposons could be the source of variation because of their genome plasticity nature.

Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium

B. H. Wang, Rong, P., Cai, X. X., Wang, W., Zhu, X. Y., Chen, C. J., Xu, Y. Y., Huang, X. J., Zhuang, Z. M., and Wang, C. B., Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium, vol. 14, pp. 10630-10644, 2015.

Cotton (Gossypium spp) is one of the most economically important crops that provide the world’s most widely used natural fiber. Diseases such as Fusarium wilt and particularly Verticillium wilt seriously affect cotton production, and thus breeding for disease resistance is one of the most important goals of cotton breeding programs. Currently, potential exists to improve disease resistance in cultivated cotton.

Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb

W. H. Zheng, Zhuo, Y., Liang, L., Ding, W. Y., Liang, L. Y., and Wang, X. F., Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb, vol. 14, pp. 10422-10432, 2015.

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of C. wenyujin, using ran­dom amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers.

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