Genetic diversity

Genetic diversity in mutated and non-mutated rice varieties

M. Naeem, Ghouri, F., Shahid, M. Q., Iqbal, M., Baloch, F. S., Chen, L., Allah, S., Babar, M., and Rana, M., Genetic diversity in mutated and non-mutated rice varieties, vol. 14, pp. 17109-17123, 2015.

We studied the genetic variability due to mutation induced by γ-rays (10, 15, and 20 Kr) on various traits of twelve rice genotypes. Mutated and non-mutated seeds were sown in the field between July 2013 and 2014 using a split plot design. Yield and yield-related trait data was recorded, which showed significant (P

Genetic characterization of red-colored heartwood genotypes of Chinese fir using simple sequence repeat (SSR) markers

H. J. Duan, Hu, R. Y., Wu, B., Chen, D. X., Huang, K. Y., Dai, J., Chen, Q., Wei, Z. C., Cao, S., Sun, Y. H., and Li, Y., Genetic characterization of red-colored heartwood genotypes of Chinese fir using simple sequence repeat (SSR) markers, vol. 14, pp. 18552-18561, 2015.

The present study investigated the genetic characterization of red-colored heartwood Chinese fir [Cunninghamia lanceolata (Lamb.) Hook.] in Guangxi using 21 simple sequence repeat (SSR) markers and analyzes of the genetic variation (N = 149) in samples obtained from five sites in Guangxi Province, China. The number of different alleles and the Shannon’s information index per locus ranged from 3 to 12 and from 0.398 to 2.258 with average values of 6 and 1.211, respectively, indicating moderate levels of genetic diversity within this germplasm collection.

Genetic variation in wild populations of the tuber crop Amorphophallus konjac (Araceae) in central China as revealed by AFLP markers

C. Pan, Gichira, A. W., and Chen, J. M., Genetic variation in wild populations of the tuber crop Amorphophallus konjac (Araceae) in central China as revealed by AFLP markers, vol. 14, pp. 18753-18763, 2015.

Amorphophallus konjac is an economically important crop. In order to provide baseline information for sustainable development and conservation of the wild plant resources of A. konjac, we studied the genetic diversity and population structure of this species using amplified fragment length polymorphism (AFLP) molecular markers. We sampled 139 individuals from 10 wild populations of A. konjac in central China. Using five AFLP primer combinations, we scored a total of 270 DNA fragments, most of which were polymorphic (98.2%).

Genetic analysis of five sedentary fish species in middle Laranjinha River (upper Paraná River basin): A case study

W. Frantine-Silva, Ferreira, D. G., Nascimento, R. H. C., Fracasso, J. F., Conte, J. E., Ramos, F. P., Carvalho, S., and Galindo, B. A., Genetic analysis of five sedentary fish species in middle Laranjinha River (upper Paraná River basin): A case study, vol. 14, pp. 18637-18649, 2015.

Most studies of diversity and genetic structure in neotropical fish have focused on commercial species from large rivers or their reservoirs. However, smaller tributaries have been identified as an important alternative migratory route, with independent pools of genetic diversity. In this context, the present study aimed to evaluate genetic diversity and structure in five neotropical fish species from a region of Laranjinha River in the upper Paraná River basin.

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

S. Ghariani, Elazreg, H., Chtourou-Ghorbel, N., Chakroun, M., and Trifi-Farah, N., Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers, vol. 14, pp. 18523-18533, 2015.

Tunisia is rich in diverse forage and pasture species including perennial ryegrass. In order to enhance forage production and improve agronomic performance of this local germplasm, a molecular analysis was undertaken. Random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and morpho-agronomical traits markers were used for genetic diversity estimation of ryegrass germplasm after screening 20 spontaneous accessions, including a local and an introduced cultivars.

Microsatellite DNA markers and their correlation with growth traits in mandarin fish (Siniperca chuatsi)

L. F. Sun, Li, J., Liang, X. F., Yi, T. L., Fang, L., Sun, J., He, Y. H., Luo, X. N., Dou, Y. Q., and Yang, M., Microsatellite DNA markers and their correlation with growth traits in mandarin fish (Siniperca chuatsi), vol. 14, pp. 19128-19135, 2015.

The mandarin fish (Siniperca chuatsi) is a traditionally cultured freshwater fish with high commercial value in China. To facilitate marker-assisted selection in genetic improvement of this species, 120 microsatellite markers from the literature were characterized in the 25 largest and 25 smallest individuals. Eighteen polymorphic loci were then used to genotype 200 individuals, and the associations between their genotypes and growth traits were examined.

Assessment of genetic diversity of cucumber cultivars in China based on simple sequence repeats and fruit traits

Y. T. Yang, Liu, Y., Qi, F., Xu, L. L., Li, X. Z., Cong, L. J., Guo, X., Chen, S. X., and Fang, Y. L., Assessment of genetic diversity of cucumber cultivars in China based on simple sequence repeats and fruit traits, vol. 14, pp. 19028-19039, 2015.

The cucumber (Cucumis sativus L.) is an important crop grown worldwide. In this study, the genetic diversity of 42 cucumber cultivars in China was analyzed using 51 pairs of simple sequence repeat (SSR) primers. These primers identified 129 polymorphic loci, 95.6% of which were polymorphic. The mean effective number of alleles, mean Nei’s gene diversity, and mean Shannon’s information index were 0.36, 0.16, and 0.21, respectively.

Functional molecular markers (EST-SSR) in the full-sib reciprocal recurrent selection program of maize (Zea mays L.)

K. S. C. Galvão, Ramos, H. C. C., Santos, P. H. A. D., Entringer, G. C., Vettorazzi, J. C. F., and Pereira, M. G., Functional molecular markers (EST-SSR) in the full-sib reciprocal recurrent selection program of maize (Zea mays L.), vol. 14, pp. 7344-7355, 2015.

This study aimed to improve grain yield in the full-sib reciprocal recurrent selection program of maize from the North Flumi­nense State University. In the current phase of the program, the goal is to maintain, or even increase, the genetic variability within and among populations, in order to increase heterosis of the 13th cycle of reciprocal recurrent selection. Microsatellite expressed sequence tags (EST-SSRs) were used as a tool to assist the maximization step of genetic variability, targeting the functional genome.

Genetic diversity in intraspecific hybrid populations of Eucommia ulmoides Oliver evaluated from ISSR and SRAP molecular marker analysis

J. Yu, Wang, Y., Ru, M., Peng, L., and Liang, Z. S., Genetic diversity in intraspecific hybrid populations of Eucommia ulmoides Oliver evaluated from ISSR and SRAP molecular marker analysis, vol. 14, pp. 7417-7425, 2015.

Eucommia ulmoides Oliver, the only extant species of Eucommiaceae, is a second-category state-protected endangered plant in China. Evaluation of genetic diversity among some intraspecific hybrid populations of E. ulmoides Oliver is vital for breeding programs and further conservation of this rare species. We studied the genetic diversity of 130 accessions from 13 E. ulmoides intraspecific hybrid populations using inter-simple sequence related (ISSR) and sequence-related amplified polymorphism (SRAP) markers.

Genetic diversity, population structure, and association mapping of agronomic traits in waxy and normal maize inbred lines

K. J. Sa, Park, J. Y., Choi, S. H., Kim, B. W., Park, K. J., and Lee, J. K., Genetic diversity, population structure, and association mapping of agronomic traits in waxy and normal maize inbred lines, vol. 14, pp. 7502-7518, 2015.

Understanding genetic diversity, population structure, and linkage disequilibrium is a prerequisite for the association mapping of complex traits in a target population. In this study, the genetic diversity and population structure of 40 waxy and 40 normal inbred maize lines were investigated using 10 morphological traits and 200 simple sequence repeat (SSR) markers. Based on a population structure analysis, the 80 maize inbred lines were divided into three groups: I, II, and admixed.

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