Genetic diversity

Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae)

J. M. Lai, Tsai, C. C., Yen, C. R., Ko, Y. Z., Chen, S. R., Weng, I. S., Lin, Y. S., and Chiang, Y. C., Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae), vol. 14, pp. 13013-13021, 2015.

Syzygium samarangense (Blume) Merr. & Perry (wax apple) is an important commercial fruit tree in Southeast Asia. Here, microsatellite markers were developed to evaluate genetic diversity and distinguish cultivars in this species. In total, 161 microsatellite loci with sufficient flanking sequences to design primer sets were isolated from wax apple using a magnetic bead-enrichment method. Fifty-eight primer sets were designed based on the flanking sequences of each single sequence repeat (SSR) locus and were tested using 14 wax apple cultivars/lines.

Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers

L. Wei, Shao, W. W., Zhou, H. B., Ping, J., Li, L. M., and Zhang, Y. P., Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers, vol. 14, pp. 14119-14122, 2015.

Twelve polymorphic microsatellite loci were isolated in the Japanese gecko, Gekko japonicus. We genotyped one population from Wenzhou, Zhejiang Province, China (N = 36). The mean number of observed alleles per locus was 7.3 (range 4 to 13). Observed and expected heterozygosity values ranged from 0.200 to 0.944 and from 0.395 to 0.797, respectively. One locus (GJ20) showed significant departure from Hardy-Weinberg equilibrium; no linkage disequilibrium was found between any two loci.

Genetic diversity and relationship analysis of Gossypium arboreum accessions

F. Liu, Zhou, Z. L., Wang, C. Y., Wang, Y. H., Cai, X. Y., Wang, X. X., Zhang, Z. S., and Wang, K. B., Genetic diversity and relationship analysis of Gossypium arboreum accessions, vol. 14, pp. 14522-14529, 2015.

Simple sequence repeat techniques were used to identify the genetic diversity of 101 Gossypium arboreum accessions collected from India, Vietnam, and the southwest of China (Guizhou, Guangxi, and Yunnan provinces). Twenty-six pairs of SSR primers produced a total of 103 polymorphic loci with an average of 3.96 polymorphic loci per primer. The average of the effective number of alleles, Nei’s gene diversity, and Shannon’s information index were 0.59, 0.2835, and 0.4361, respectively. The diversity varied among different geographic regions.

Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species

Q. H. Gu, Cheng, Q. Q., Li, X. J., and Zhou, C. J., Novel polymorphic microsatellite markers for Bellamya and their application in population genetics of three species, vol. 14, pp. 15201-15212, 2015.

Bellamya is a widely distributed freshwater snail genus in China; however, its genetic diversity is completely unknown. Sixty-five novel microsatellite loci were isolated and characterized from a microsatellite-enriched library of Bellamya aeruginosa genomic DNA. Most of the 65 loci were successfully amplified. We found high polymorphic information content values for these loci, ranging from 0.235 to 0.892. There were 3 to 12 alleles per locus, and the HE and HO varied from 0.425 to 0.953 and 0.026 to 1.000, respectively.

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

G. A. Luz, Gomes, S. O., Neto, R. B. Araújo, Nascimento, M. S. C. B., and Lima, P. S. C., Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers, vol. 14, pp. 15242-15248, 2015.

Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence.

Genetic diversity in three natural populations of Pitcairnia flammea (l.) John (Bromeliaceae) estimated by ISSR markers

F. B. Souza-Sobreira, Souza, G. B., Rosado, C. C. G., Miranda, F. D., Soares, T. C. B., and Gontijo, A. B. P. L., Genetic diversity in three natural populations of Pitcairnia flammea (l.) John (Bromeliaceae) estimated by ISSR markers, vol. 14, pp. 15892-15901, 2015.

Bromeliads are greatly represented in the Atlantic Forest, although many species are threatened with extinction owing to habitat fragmentation and intense extraction for ornamental purposes. Therefore, it is necessary to conduct studies generating knowledge about genetic diversity and the distribution of this diversity among and within natural populations to establish conservation strategies. These studies can be performed with the use of molecular markers.

Genetic diversity analysis of fruit characteristics of hawthorn germplasm

K. Su, Guo, Y. S., Wang, G., Zhao, Y. H., and Dong, W. X., Genetic diversity analysis of fruit characteristics of hawthorn germplasm, vol. 14, pp. 16012-16017, 2015.

One hundred and six accessions of hawthorn intraspecific resources, from the National Germplasm Repository at Shenyang, were subjected to genetic diversity and principal component analysis based on evaluation data of 15 fruit traits. Results showed that the genetic diversity of hawthorn fruit traits varied. Among the 15 traits, the fruit shape variable coefficient had the most obvious evaluation, followed by fruit surface state, dot color, taste, weight of single fruit, sepal posture, peduncle form, and metula traits.

Genetic diversity analysis of Capparis spinosa L. populations by using ISSR markers

C. Liu, Xue, G. P., Cheng, B., Wang, X., He, J., Liu, G. H., and Yang, W. J., Genetic diversity analysis of Capparis spinosa L. populations by using ISSR markers, vol. 14, pp. 16476-16483, 2015.

Capparis spinosa L. is an important medicinal species in the Xinjiang Province of China. Ten natural populations of C. spinosa from 3 locations in North, Central, and South Xinjiang were studied using morphological trait inter simple sequence repeat (ISSR) molecular markers to assess the genetic diversity and population structure. In this study, the 10 ISSR primers produced 313 amplified DNA fragments, with 52% of fragments being polymorphic. Unweighted pair-group method with arithmetic average (UPGMA) cluster analysis indicated that 10 C.

Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome

E. R. Monteiro, Strioto, D. K., Meirelles, A. C. S., Mangolin, C. A., and Machado, M. F. P. S., Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome, vol. 14, pp. 16966-16974, 2015.

Amplified fragment length polymorphism (AFLP) analysis was used to evaluate DNA polymorphism in Pilosocereus gounellei with the aim of differentiating samples grown in different Brazilian semiarid regions. Seven primer pairs were used to amplify 703 AFLP markers, of which 700 (99.21%) markers were polymorphic. The percentage of polymorphic markers ranged from 95.3% for the primer combination E-AAG/M-CTT to 100% for E-ACC/M-CAT, E-ACC/M-CAA, E-AGC/M-CAG, E-ACT/M-CTA, and E-AGG/M-CTG.

Population genetic structure of Myzus persicae nicotianae (Hemiptera: Aphididae) in China by microsatellite analysis

C. Zhao, Yang, X. M., Tang, S. H., Xu, P. J., Bian, W. J., Wang, X. F., Wang, X. W., and Ren, G. W., Population genetic structure of Myzus persicae nicotianae (Hemiptera: Aphididae) in China by microsatellite analysis, vol. 14, pp. 17159-17169, 2015.

The tobacco aphid, Myzus persicae nicotianae (Hemiptera: Aphididae), is an important agricultural pest that feeds on host plants and transmits plant viruses in China. To effectively control this pest, we investigated the genetic variation and genetic structure of 54 populations of tobacco aphids collected in China, using five microsatellite loci.

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