Genetic diversity

Validation of EST-derived microsatellite markers for two Cerrado-endemic Campomanesia (Myrtaceae) species

E. A. G. C. Miranda, Boaventura-Novaes, C. R. D., Braga, R. S., Reis, E. F., Pinto, J. F. N., Telles, M. P. C., Miranda, E. A. G. C., Boaventura-Novaes, C. R. D., Braga, R. S., Reis, E. F., Pinto, J. F. N., and Telles, M. P. C., Validation of EST-derived microsatellite markers for two Cerrado-endemic Campomanesia (Myrtaceae) species, vol. 15. p. -, 2016.

We assessed the transferability of 120 EST-derived Eucalyptus microsatellite primers to Campomanesia adamantium and C. pubescens. Both species are berry trees native to the Brazilian Cerrado, and population genetic information is poor. Twelve markers were used to analyze the genetic variability of four sampled populations. Regarding DNA extraction, we sampled leaf tissues from two populations of each species (80 individuals). Of the 120 primers evaluated, 87 did not amplify any PCR products, and 21 rendered nonspecific amplification.

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