genetic breeding

Selfing confirmation in sugarcane by using simple sequence repeat markers: an individual reciprocal recurrent selection scheme

P. M. A. Costa, Almeida, C. F., Silveira, G., Soares, B., Baffa, D. C. F., Peternelli, L. A., Bhering, L. L., and Barbosa, M. H. P., “Selfing confirmation in sugarcane by using simple sequence repeat markers: an individual reciprocal recurrent selection scheme”, vol. 13, pp. 8962-8970, 2014.

Superior inbred clones selected in S1 families can integrate an individual reciprocal recurrent selection program in sugarcane by eliminating the genetic load of the population and exploring superior hybrid combinations. Molecular markers can be used for reliable identification of the true selfing-derived clones in these S1 populations.

Genetic variation in a wild population of the ‘sleep’ passion fruit (Passiflora setacea) based on molecular markers

C. B. M. Cerqueira-Silva, Santos, E. S. L., Conceição, L. D. H. C. S., Cardoso-Silva, C. B., Pereira, A. S., Oliveira, A. C., and Corrêa, R. X., “Genetic variation in a wild population of the ‘sleep’ passion fruit (Passiflora setacea) based on molecular markers”, vol. 11. pp. 731-738, 2012.

Little is known about the molecular genetic diversity of most Passiflora species. We used RAPD markers to evaluate the genetic diversity of 24 genotypes of the ‘sleep’ passion fruit (Passiflora setacea). Twelve primers generated 95 markers, 88% of which were polymorphic. The genetic distance estimated by the complement of the Dice index ranged from 0.29 (among accessions Ps-G1 and Ps-G13) to 0.69 (among accessions Ps-G21 and Ps-G23). Genotype grouping based on the UPGMA algorithm showed considerable variability among genotypes. We conclude that P.

New microsatellite markers for bananas (Musa spp)

E. P. Amorim, Silva, P. H., Ferreira, C. F., Amorim, V. B. O., Santos, V. J., Vilarinhos, A. D., Santos, C. M. R., Júnior, M. T. Souza, and Miller, R. N. G., “New microsatellite markers for bananas (Musa spp)”, vol. 11. pp. 1093-1098, 2012.

Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly informative based on their polymorphism information content values; more than 50% were above 0.5.

Genetic diversity in wild species of passion fruit (Passiflora trintae) based on molecular markers

C. B. M. Cerqueira-Silva, Cardoso-Silva, C. B., Santos, E. S. L., Conceição, L. D. H. C. S., Pereira, A. S., Oliveira, A. C., and Corrêa, R. X., “Genetic diversity in wild species of passion fruit (Passiflora trintae) based on molecular markers”, vol. 9, pp. 2123-2130, 2010.

In spite of the importance of and the considerable variability observed in Passiflora (Passifloraceae), little is known about the genetic diversity of most of the species of this genus. We evaluated the genetic diversity by RAPD markers in 18 genotypes of Passiflora trintae. The 15 primers generated 112 markers, 84% of which were polymorphic. The genetic distance estimated by the complement of the Dice index (average dissimilarity = 0.30) and genotype grouping based on the UPGMA algorithm showed low variability among genotypes.

Genetic variability in wild genotypes of Passiflora cincinnata based on RAPD markers

C. B. M. Cerqueira-Silva, Conceição, L. D. H. C. S., Santos, E. S. L., Cardoso-Silva, C. B., Pereira, A. S., Oliveira, A. C., and Corrêa, R. X., “Genetic variability in wild genotypes of Passiflora cincinnata based on RAPD markers”, vol. 9, pp. 2421-2428, 2010.

The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected from the wild in the region of Vitória da Conquista, Bahia, Brazil. Thirteen primers generated 95 polymorphic markers and only one monomorphic marker.

Backcross assisted by microsatellite markers in common bean

L. K. Oliveira, Melo, L. C., Brondani, C., Peloso, M. J. D., and Brondani, R. P. V., “Backcross assisted by microsatellite markers in common bean”, vol. 7, pp. 1000-1010, 2008.

The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virus and, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed.

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