Gene function

Molecular characterization, expression patterns, and promoter activity analysis of PGM1 in pigs

J. Y. Yu, Shao, S. M., and Xiong, Y. Z., Molecular characterization, expression patterns, and promoter activity analysis of PGM1 in pigs, vol. 14, pp. 2816-2824, 2015.

The phosphoglucomutase 1 (PGM1) gene was differentially expressed in tissues of Chinese Meishan and Large White pigs. In this study, the promoter region, expression profile, and genetic mutations of the gene were determined. Expression of a 5'-deletion in both C2C12 and PK-15 cells showed that a negative regulatory element was at -1871 to +185 bp and a positive regulatory element was at -1158 to +185 bp. Among the different types of muscle fibers, PGM1 had the highest expression in both longissimus dorsi and biceps femoris.

Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system

M. L. Liao, Peng, Z. S., Yang, Z. J., Wei, S. H., and Martinek, P., Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system, vol. 14, pp. 3995-4004, 2015.

HTS-1 is a new kind of pistillody wheat. All or parts of its stamen are transformed into pistils or pistil-like structures, and it has more seed sets per floret than normal wheat under normal cultivation conditions. To investigate the expression divergence in this mutant, an annealing control primer system was used to identify differentially expressed genes (DEGs) in the young spikelets. As a result, three DEGs, including HDB2, HGF2, and HCG4, were detected, with variable expression in HTS-1 and the control.

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