Gene expression

Selection of reference genes for expression analyses of red-fleshed sweet orange (Citrus sinensis)

T. T. Pinheiro, Nishimura, D. S., De Nadai, F. B., Figueira, A., and Latado, R. R., Selection of reference genes for expression analyses of red-fleshed sweet orange (Citrus sinensis), vol. 14, pp. 18440-18451, 2015.

Red-fleshed oranges (Citrus sinensis) contain high levels of carotenoids and lycopene. The growing consumer demand for products with health benefits has increased interest in these types of Citrus cultivars as a potential source of nutraceuticals. However, little is known about the physiology of these cultivars under Brazilian conditions. Transcriptome and gene expression analyses are important tools in the breeding and management of red-fleshed sweet orange cultivars.

Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids

M. Cervantes, Arce, N., García, H., Cota, M., Htoo, J. K., and Morales, A., Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids, vol. 14, pp. 9779-9792, 2015.

Excess Leu reduces the availability of Ile and Val in pigs likely by reducing absorption of the latter amino acids (AA). Twenty-four crossbred pigs were used to evaluate the effect of excess Leu alone or with surplus Ile and Val on the expression of cationic (b0,+AT and CAT1) and neutral (B0AT1) AA transporters in the small intestine, liver, and skeletal muscle. Dietary treatments included: 1) basal diet; 2) basal plus 0.43% L-Leu (excess Leu); 3) basal plus 0.43% Leu, 0.20% L-Ile, and 0.25% L-Val (excess Leu, Ile, and Val).

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