Gene cloning

Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa

B. L. Huang, Cheng, C., Zhang, G. Y., Su, J. J., Zhi, Y., Xu, S. S., Cai, D. T., Zhang, X. K., and Huang, B. Q., Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa, vol. 14, pp. 18121-18130, 2015.

Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. In this study, a gene encoding EvNAS was cloned and characterized in E. vesicaria subsp sativa.

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