Gene cloning

Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease

C. H. Shan, Tang, F. X., Chen, W., and Ma, W. R., Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease, vol. 14, pp. 18229-18240, 2015.

Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the cloned gene fragment was confirmed to be the Hami melon Cu/Zn-SOD gene sequence.

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