Gene clone

Molecular cloning and expression analysis of a matrix Gla protein gene in the spinyhead croaker, Collichthys lucidus

W. Song, Zhao, M. D., Jiang, K. J., Zhang, F. Y., Zhao, M., Meng, Y. Y., Ma, L. B., Song, W., Zhao, M. D., Jiang, K. J., Zhang, F. Y., Zhao, M., Meng, Y. Y., and Ma, L. B., “Molecular cloning and expression analysis of a matrix Gla protein gene in the spinyhead croaker, Collichthys lucidus”, vol. 15, no. 4, p. -, 2016.

INTRODUCTION

Molecular cloning and expression analyses of RPS3a gene from mulberry under abiotic stresses and among different mulberry varieties

J. Qian, Zhou, H., Zhao, M. D., Wang, H., Li, F., Wang, Y. H., Fang, R. J., Zhao, W. G., Kim, H. J., Qian, J., Zhou, H., Zhao, M. D., Wang, H., Li, F., Wang, Y. H., Fang, R. J., Zhao, W. G., and Kim, H. J., “Molecular cloning and expression analyses of RPS3a gene from mulberry under abiotic stresses and among different mulberry varieties”, vol. 15, p. -, 2016.

A full-length cDNA sequence coding ribosomal protein S3a of mulberry tree, which we designated MmRPS3a (GenBank accession No. KR610331), was cloned based on mulberry expressed sequence tags. Sequence analysis showed that the MmRPS3a is 1089 bp long and contains a 80-bp 5'-UTR (untranslated region) and a 220-bp 3'-UTR. Its open reading frame consists of a 789-bp encoding 262 amino acids with a predicted molecular weight of 30.053 kDa and an isoelectric point of 9.84.

Molecular cloning and mRNA expression of a hepcidin gene from the spinyhead croaker, Collichthys lucidus

C. Sang, Lin, Y., Jiang, K., Zhang, F., and Song, W., “Molecular cloning and mRNA expression of a hepcidin gene from the spinyhead croaker, Collichthys lucidus”, vol. 14, pp. 16050-16059, 2015.

Antimicrobial peptides are important components that participate in host innate immune activities and play crucial roles in host defense against microbial invasion. Hepcidin is an antimicrobial peptide and iron-regulatory molecule that primarily functions in the liver. In the present study, we first obtained a full-length cDNA sequence of hepcidin and its corresponding genomic DNA sequence from Collichthys lucidus using RT-PCR and rapid amplification of cDNA ends (RACE), and then analyzed these sequences using bioinformatics software. The results showed that C.

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