Gene activation

Hepatitis B virus X protein activates human hepatic stellate cells through upregulating TGFβ1

H. - Y. Chen, Chen, Z. - X., Huang, R. - F., Lin, N., and Wang, X. - Z., Hepatitis B virus X protein activates human hepatic stellate cells through upregulating TGFβ1, vol. 13, pp. 8645-8656, 2014.

We investigated the effects of the hepatitis B virus X gene (HBV X) on the activation of human hepatic stellate cells (HSCs) and the possible mechanisms underlying the pathway. Recombinant plasmid pHBV-X-IRES2-EGFP was constructed and transfected into HL-7702 cells using a lipid-mediated method. Transfected cells were screened by G418, which detected stable expression of the X gene by reverse transcription (RT)-PCR and Western blot analysis, and named L02/x.

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