Hepatitis B virus X protein activates human hepatic stellate cells through upregulating TGFβ1
“Hepatitis B virus X protein activates human hepatic stellate cells through upregulating TGFβ1”, vol. 13, pp. 8645-8656, 2014.
, We investigated the effects of the hepatitis B virus X gene (HBV X) on the activation of human hepatic stellate cells (HSCs) and the possible mechanisms underlying the pathway. Recombinant plasmid pHBV-X-IRES2-EGFP was constructed and transfected into HL-7702 cells using a lipid-mediated method. Transfected cells were screened by G418, which detected stable expression of the X gene by reverse transcription (RT)-PCR and Western blot analysis, and named L02/x.