Gastric cancer cells

Screening for genes that are differentially-expressed between gastric cancer cells and gastric tumor sphere cells using the gene chip technique

W. H. Shi, Yu, Y. B., Li, Z. - Y., Guo, X. - C., Wei, Z. - L., and Zheng, Z. - H., Screening for genes that are differentially-expressed between gastric cancer cells and gastric tumor sphere cells using the gene chip technique, vol. 14, pp. 14893-14899, 2015.

The purpose of this study was to screen for genes that were differentially expressed between a human gastric carcinoma cell line (HGC-27) and their tumor spheres, using the gene chip technique. The HGC-27 cells and tumor sphere cells were cultured in vitro in a sterile environment. Total RNA was extracted from both samples and purified using a standard TRIzol reagent. Total RNA was then hybridized onto a GeneChip, according to the standard protocols provided by the manufacturers of the GeneChip IVT Express Kit.

Effects of simulated weightlessness on cellular morphology and biological characteristics of cell lines SGC-7901 and HFE-145

M. Zhu, Jin, X. W., Wu, B. Y., Nie, J. L., and Li, Y. H., Effects of simulated weightlessness on cellular morphology and biological characteristics of cell lines SGC-7901 and HFE-145, vol. 13, pp. 6060-6069, 2014.

We investigated the effects of simulated weightlessness on cellular morphology, proliferation, cell cycle, and apoptosis of the human gastric carcinoma cell line SGC-7901 and the human gastric normal cell line HFE-145. A rotating clinostat was used to simulate weightlessness. The Image-Pro4.5 image analysis system was used for morphometric analysis. Proliferating cell nuclear antigen expression was examined by immunohistochemical staining. Changes in the cell cycle were examined using a cytometer. Apoptosis was measured using the terminal dUTP nick-end labeling (TUNEL) method.

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