Fungi

An economical and combined method for rapid and efficient isolation of fungal DNA

T. Lech, Syguła-Cholewinska, J., and Szostak-Kot, J., An economical and combined method for rapid and efficient isolation of fungal DNA, vol. 13, pp. 10779-10786, 2014.

DNA isolation is a crucial step of conducting genetic studies in any organism. However, this process is quite difficult when studying fungi because of the need to damage the fungal cell walls of specific structures. In this study, we developed a method for the rapid and efficient isolation of fungal DNA based on simultaneous mechanical and enzymatic cell wall degradation. There are several typical modifications of the standard phenol-chloroform DNA extraction method. This method can be modified to degrade the fungal cell wall.

Therapeutic targets in Paracoccidioides brasiliensis: post-transcriptome perspectives

L. Fernandes, Galdino, A. Sobreira, Felipe, M. Sueli Soar, Soares, C. Maria de A., Pereira, M., and Amaral, A. Corrêa, Therapeutic targets in Paracoccidioides brasiliensis: post-transcriptome perspectives, vol. 4, pp. 430-449, 2005.

The rise in antifungal resistance, observed as a result of the increasing numbers of immunocompromised patients, has made the discovery of new targets for drug therapy imperative. The description of the Paracoccidioides brasiliensis transcriptome has allowed us to find alternatives to refine current therapy against paracoccidioidomycosis. We used comparative analysis of expressed sequence tags to find promising drug targets that have been addressed in other pathogens.

Cell signaling pathways in Paracoccidioides brasiliensis - inferred from comparisons with other fungi

L. Fernandes, Araújo, M. A. M., Amaral, A., Reis, V. Castelo Br, Martins, N. F., and Felipe, M. S., Cell signaling pathways in Paracoccidioides brasiliensis - inferred from comparisons with other fungi, vol. 4, pp. 216-231, 2005.

The human fungal pathogen Paracoccidioides brasiliensis is an ascomycete that displays a temperature-dependent dimorphic transition, appearing as a mycelium at 22°C and as a yeast at 37°C, this latter being the virulent form. We report on the in silico search made of the P.

Sex in fungi: lessons of gene regulation

C. A. J. Souza, Silva, C. C., and Ferreira, A. V. - B., Sex in fungi: lessons of gene regulation, vol. 2. pp. 136-147, 2003.

Fungi have been very useful for gene regulation studies. Mating implicates in a series of events influenced by many types of environmental input that are interpreted into regulatory pathways, including signal transduction. Although various aspects of mating and signal transduction in the yeast Saccharomyces cerevisiae have long been characterized, recent findings in filamentous fungi indicate that pheromones and pheromone receptors may be essential for mating partner recognition and also for nucleus recognition in sorting before meiosis.

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